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IHC (Immunohistchemistry) (AIFM1/AIF/PDCD8 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Goat anti-Human AIFM1/AIF/PDCD8 Polyclonal Antibody | anti-AIFM1 antibody

AIFM1/AIF/PDCD8 Goat anti-Human Polyclonal Antibody

Gene Names
AIFM1; AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
AIFM1/AIF/PDCD8; Polyclonal Antibody; AIFM1/AIF/PDCD8 Goat anti-Human Polyclonal Antibody; IHC-plus AIFM1/AIF/PDCD8 Antibody; AIFM1; AIF; COXPD6; PDCD8; Programmed cell death 8; anti-AIFM1 antibody
Ordering
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
TBS, pH7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-AIFM1 antibody
Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)
Target
Human AIFM1/AIF/PDCD8
Immunogen
Peptide with sequence C-NEVAKLFNIHED, from the C-Terminus of protein sequence according to NP_004199.1NP_665811.1NP_665812.1NP_001124318.1.
Conjugation
Unconjugated
Family
Apoptosis
Preparation and Storage
Aliquot and store at -20 degree C. Avoid freeze-thaw cycles.

IHC (Immunohistchemistry)

(AIFM1/AIF/PDCD8 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (AIFM1/AIF/PDCD8 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(AIFM1/AIF/PDCD8 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (AIFM1/AIF/PDCD8 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(AIFM1/AIF/PDCD8 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (AIFM1/AIF/PDCD8 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.01ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.01ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

WB (Western Blot)

(AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.01ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.01ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

WB (Western Blot)

(AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.)

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.)

WB (Western Blot)

(AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.03ug/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.03ug/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

ICC (Immunocytochemistry)

(AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

ICC (Immunocytochemistry) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

ICC (Immunocytochemistry)

(AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

ICC (Immunocytochemistry) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Related Product Information for anti-AIFM1 antibody
PDCD8 antibody is an unconjugated goat polyclonal antibody to human PDCD8 (AIFM1/AIF). Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,033 Da
NCBI Official Full Name
apoptosis-inducing factor 1, mitochondrial isoform AIF
NCBI Official Synonym Full Names
apoptosis inducing factor mitochondria associated 1
NCBI Official Symbol
AIFM1
NCBI Official Synonym Symbols
AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6
NCBI Protein Information
apoptosis-inducing factor 1, mitochondrial
UniProt Protein Name
Apoptosis-inducing factor 1, mitochondrial
UniProt Gene Name
AIFM1
UniProt Synonym Gene Names
AIF; PDCD8
UniProt Entry Name
AIFM1_HUMAN

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Product Notes

The AIFM1 aifm1 (Catalog #AAA21377) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The AIFM1/AIF/PDCD8 Goat anti-Human Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AIFM1/AIF/PDCD8 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the AIFM1 aifm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIFM1/AIF/PDCD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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