AIFM1 recombinant protein

AIFM1, 98-609aa, Human, His tag, E Coli

Cat. Num. AAA11826

• Gene Names: AIFM1; AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: AIFM1; N/A; AIFM1, 98-609aa, Human, His tag, E Coli; Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor; Apoptosis-inducing factor 1; mitochondrial isoform 2 precursor; AIF; CMTX4; COWCK; COXPD6; PDCD8; AIFM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFLGLTP EQKQKKAALS ASEGEEVPQD KAPSHVPFLL IGGGTAAFAA ARSIRARDPG ARVLIVSEDP ELPYMRPPLS KELWFSDDPN VTKTLRFKQW NGKERSIYFQ PPSFYVSAQD LPHIENGGVA VLTGKKVVQL DVRDNMVKLN DGSQITYEKC LIATGGTPRS LSAIDRAGAE VKSRTTLFRK IGDFRSLEKI SREVKSITII GGGFLGSELA CALGRKARAL GTEVIQLFPE KGNMGKILPE YLSNWTMEKV RREGVKVMPN AIVQSVGVSS GKLLIKLKDG RKVETDHIVA AVGLEPNVEL AKTGGLEIDS DFGGFRVNAE LQARSNIWVA GDAACFYDIK LGRRRVEHHD HAVVSGRLAG ENMTGAAKPY WHQSMFWSDL GPDVGYEAIG LVDSSLPTVG VFAKATAQDN PKSATEQSGT GIRSESETES EASEITIPPS TPAVPQAPVQ GEDYGKGVIF YLRDKVVVGI VLWNIFNRMP IARKIIKDGE QHEDLNEVAK LFNIHED
• Sequence Length: 609

• Applicable Applications for AIFM1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-PAGE

Related Product Information for AIFM1 recombinant protein

Apoptosis-inducing factor 1, mitochondrial isoform 2 precursor, also known as AIFM1, is a mitochondrial protein that translocates to the nucleus upon induction of apoptosis. AIFM1 has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIFM1 from mitochondria, but not to block its apoptogenic activity. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Recombinant human AIFM1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for AIFM1 recombinant protein

Metabolism

References

Zamzami N., et al. (1996) J Exp Med. 183: 1533-1544.; Susin S A., et al. (1996) J Exp Med. 184: 1331-1341.;

NCBI and Uniprot Product Information

• NCBI GI #: 22202629
• NCBI GeneID: 9131
• NCBI Accession #: NP_665811
• NCBI GenBank Nucleotide #: NM_145812.2
• UniProt Accession #: O95831; Q1L6K4; Q1L6K6; Q2QKE4; Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4; A4QPB4; B1ALN1; B2RB08; D3DTE9
• Molecular Weight: 58.5 kDa (537aa)
• NCBI Official Full Name: apoptosis-inducing factor 1, mitochondrial isoform AIF-exB
• NCBI Official Synonym Full Names: apoptosis inducing factor mitochondria associated 1
• NCBI Official Symbol: AIFM1
• NCBI Official Synonym Symbols: AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• UniProt Gene Name: AIFM1
• UniProt Synonym Gene Names: AIF; PDCD8