Standard Curve (Sample)
Human ADAM10 ELISA Kit | ADAM10 elisa kit
Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit
Gene Names
ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
Reactivity
Human
Synonyms
ADAM10; Human ADAM10 (A Disintegrin And Metalloprotease 10) ELISA Kit; ADAM10 elisa kit
Reactivity
Human
Specificity
This kit recognizes Human ACV-A in samples. No significant cross-reactivity or interference between Human ACV-A and analogues was observed.
Sequence Length
748
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
78.13-5000pg/mL
Sensitivity
46.88pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ACV-A were tested 20 times on one plate, respectively
Inter-assay Precision
nter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ACV-A were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ADAM10 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ACV-A concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ACV-A. You can calculate the concentration of Human ACV-A in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Activin A is a disulphide-linked homodimer of the b-subunit of inhibin, a member of the transforming growth factor-b (TGF-b) 1 superfamily of proteins [1]. Although four activin genes have been discovered in humans, most studies have focused on the cell biology of activin A. This molecule is involved in the regulation of mitogenesis, embryogenesis, apoptosis, wound repair and other regulatory processes in vivo. Activin A has also been shown to play important roles in the pluripotency, proliferation and differentiation of embryonic stem cells, underpinning an emerging area of biomedical research. Activin A is initially produced as a large precursor molecule, with the pro-region separated from the mature region by a polybasic motif [1]. This motif is putatively cleaved in mammalian cells by subtilisin-like pro-protein convertases (SPCs), such as furin to produce the mature protein. Activin A has been purified from several sources, including porcine follicular fluid and ovine amniotic fluid [2].
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ACV-A. You can calculate the concentration of Human ACV-A in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Activin A is a disulphide-linked homodimer of the b-subunit of inhibin, a member of the transforming growth factor-b (TGF-b) 1 superfamily of proteins [1]. Although four activin genes have been discovered in humans, most studies have focused on the cell biology of activin A. This molecule is involved in the regulation of mitogenesis, embryogenesis, apoptosis, wound repair and other regulatory processes in vivo. Activin A has also been shown to play important roles in the pluripotency, proliferation and differentiation of embryonic stem cells, underpinning an emerging area of biomedical research. Activin A is initially produced as a large precursor molecule, with the pro-region separated from the mature region by a polybasic motif [1]. This motif is putatively cleaved in mammalian cells by subtilisin-like pro-protein convertases (SPCs), such as furin to produce the mature protein. Activin A has been purified from several sources, including porcine follicular fluid and ovine amniotic fluid [2].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,047 Da
NCBI Official Full Name
ADAM10
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 10
NCBI Official Symbol
ADAM10
NCBI Official Synonym Symbols
RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 10; CDw156; a disintegrin and metalloprotease domain 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
UniProt Gene Name
ADAM10
UniProt Synonym Gene Names
KUZ; MADM; ADAM 10
UniProt Entry Name
ADA10_HUMAN
Similar Products
Product Notes
The Human ADAM10 adam10 (Catalog #AAA21763) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA21763 ELISA Kit recognizes Human ADAM10. It is sometimes possible for the material contained within the vial of "ADAM10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
