Human sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ELISA Kit | SIRT1 elisa kit

Human NAD-dependent deacetylase sirtuin-1, SIRT1/SIR2L1 ELISA Kit

Cat. Num. AAA15752

• Gene Names: SIRT1; SIR2L1
• Reactivity: Human
• Synonyms: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); N/A; Human NAD-dependent deacetylase sirtuin-1, SIRT1/SIR2L1 ELISA Kit; Human NAD-dependent deacetylase sirtuin-1 (SIRT1/SIR2L1) ELISA kit; RP11-57G10.3; SIR2L1; SIR2alpha; sir2-like 1; sirtuin 1; sirtuin type 1; SIRT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SIRT1/SIR2L1. No significant cross-reactivity or interference between human SIRT1/SIR2L1 and analogues was observed.
• Sequence Length: 452
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for SIRT1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIRT1/SIR2L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRT1/SIR2L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT1/SIR2L1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT1/SIR2L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

References

Plasma levels of SIRT1 associate with non-alcoholic fatty liver disease in obese patients. Stefania Mariani, Daniela Fiore, Sabrina Basciani, Agnese Persichetti, Savina Contini, Carla Lubrano, Luisa Salvatori, Andrea Lenzi, Lucio Gnessi (2015)

NCBI and Uniprot Product Information

• NCBI GI #: 215982798
• NCBI GeneID: 23411
• NCBI Accession #: NP_001135970.1
• NCBI GenBank Nucleotide #: NM_001142498.1
• UniProt Accession #: Q96EB6; Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0
• Molecular Weight: 81,681 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-1 isoform b
• NCBI Official Synonym Full Names: sirtuin 1
• NCBI Official Symbol: SIRT1
• NCBI Official Synonym Symbols: SIR2L1
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-1
• UniProt Gene Name: SIRT1
• UniProt Synonym Gene Names: SIR2L1; hSIRT1; hSIR2; 75SirT1
• UniProt Entry Name: SIR1_HUMAN