Mouse Tight junction protein ZO-1 ELISA Kit | TJP1 elisa kit
Mouse Tight junction protein ZO-1 (TJP1) ELISA Kit
Gene Names
TJP1; ZO-1
Reactivity
Mouse
Synonyms
Tight junction protein ZO-1; N/A; Mouse Tight junction protein ZO-1 (TJP1) ELISA Kit; TJP1 elisa kit
Reactivity
Mouse
Sequence Length
1835
Assay Type
Sandwich
Detection Range
10 ng/mL-0.156 ng/mL
Sensitivity
Up to 0.05 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for TJP1 elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse TJP1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
186,966 Da
NCBI Official Full Name
tight junction protein ZO-1 isoform c
NCBI Official Synonym Full Names
tight junction protein 1
NCBI Official Symbol
TJP1
NCBI Official Synonym Symbols
ZO-1
NCBI Protein Information
tight junction protein ZO-1
UniProt Protein Name
Tight junction protein ZO-1
UniProt Gene Name
TJP1
UniProt Synonym Gene Names
ZO1
UniProt Entry Name
ZO1_HUMAN
