Standard Curve (Sample)
Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit | COL1a2 elisa kit
Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit
Gene Names
COL1A2; OI4; EDSCV; EDSARTH2
Reactivity
Human
Synonyms
Collagen Type I Alpha 2 (COL1a2); Human Collagen Type I Alpha 2 (COL1a2) ELISA Kit; COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin; Tendon And bone; Alpha-2 Chain; COL1a2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of COL1a2. No significant cross-reactivity or interference between COL1a2 and analogues was observed.
Sequence Length
23
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6-1,000pg/mL
Sensitivity
< 6.4pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL1a2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL1a2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for COL1a2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1a2 in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL1a2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL1a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL1a2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL1a2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL1a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL1a2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
References
Elafin Reverses Intestinal Fibrosis by Inhibiting Cathepsin S-Mediated Protease-Activated Receptor 2; Cell Mol Gastroenterol Hepatol; 2022;14(4):841-876.; PMID: 35840034; Ying Xie; Vatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, California; Department of Gastroenterology, The First Hospital of China Medical University, Shenyang City, Liaoning Province, China.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
2,134 Da
NCBI Official Full Name
collagen type I alpha 2, partial
NCBI Official Synonym Full Names
collagen type I alpha 2 chain
NCBI Official Symbol
COL1A2
NCBI Official Synonym Symbols
OI4; EDSCV; EDSARTH2
NCBI Protein Information
collagen alpha-2(I) chain
UniProt Protein Name
Collagen type I alpha 2
UniProt Gene Name
COL1A2
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Product Notes
The Human COL1a2 col1a2 (Catalog #AAA22916) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA22916 ELISA Kit recognizes Human COL1a2. It is sometimes possible for the material contained within the vial of "Collagen Type I Alpha 2 (COL1a2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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