Human Transient receptor potential cation channel subfamily V member 1 ELISA Kit | TRPV1 elisa kit
Human Transient receptor potential cation channel subfamily V member 1 ELISA Kit
Gene Names
TRPV1; VR1
Reactivity
Human
Synonyms
Transient receptor potential cation channel subfamily V member 1; N/A; Human Transient receptor potential cation channel subfamily V member 1 ELISA Kit; TrpV1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; TRPV1; VR1; TRPV1 elisa kit
Reactivity
Human
Specificity
Recombinant and natural Human TrpV1
Sequence Length
839
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.156-10 ng/mL
Sensitivity
<0.1 ng/mL
Intra-assay Precision
<=4.8%
Inter-assay Precision
<=7.7%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Related Product Information for TRPV1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94,211 Da
NCBI Official Full Name
transient receptor potential cation channel subfamily V member 1
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily V member 1
NCBI Official Symbol
TRPV1
NCBI Official Synonym Symbols
VR1
NCBI Protein Information
transient receptor potential cation channel subfamily V member 1
UniProt Protein Name
Transient receptor potential cation channel subfamily V member 1
UniProt Gene Name
TRPV1
UniProt Synonym Gene Names
VR1; TrpV1; OTRPC1
UniProt Entry Name
TRPV1_HUMAN