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WB (Western Blot)
(WB Suggested Anti-SQSTM1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Pancreas)
Rabbit SQSTM1 Polyclonal Antibody | anti-SQSTM1 antibody
SQSTM1 antibody - middle region
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Tested Species Reactivity: Human, MousePredicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
SQSTM1; Polyclonal Antibody; SQSTM1 antibody - middle region; anti-SQSTM1 antibody
Host
Rabbit
Reactivity
Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/mL (varies by lot)
Sequence
Synthetic peptide located within the following region: EEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQE
Sequence Length
440
Applicable Applications for anti-SQSTM1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Protein Size (# AA)
440 amino acids
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human SQSTM1
Protein Interactions
HTT; BCL2; ATXN3; MAP1LC3A; tat; GABARAPL1; GABARAPL2; KEAP1; SQSTM1; UBC; TP53; TRIM63; TRIM55; MAP1LC3B; TOLLIP; ULK1; GABARAP; OPTN; SESN2; SUMO3; NBR1; EPAS1; EGLN3; CFTR; DCP2; MGRN1; NEDD8; EDEM1; LGALS3; IKBKG; TNK2; DVL2; FLNB; PSMD12; PSMD10; PSM
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-SQSTM1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Pancreas)
WB (Western Blot)
(WB Suggested Anti-SQSTM1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Pancreas)
WB (Western Blot)
(Host: RabbitTarget Name: SQSTM1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: SQSTM1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: SQSTM1Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: SQSTM1Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: SQSTM1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: SQSTM1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: MouseTarget Name: SQSTM1Sample Tissue: Mouse BrainAntibody Dilution: 1ug/ml)
WB (Western Blot)
(Host: MouseTarget Name: SQSTM1Sample Tissue: Mouse BrainAntibody Dilution: 1ug/ml)
IHC (Immunohistochemistry)
(Rabbit Anti-SQSTM1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Thyroid Gland TissueObserved Staining: CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohistochemistry)
(Rabbit Anti-SQSTM1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Thyroid Gland TissueObserved Staining: CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohistochemistry)
(Pancreas)
IHC (Immunohistochemistry)
(Pancreas)
Related Product Information for anti-SQSTM1 antibody
This is a rabbit polyclonal antibody against SQSTM1. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to m
Target Description: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to m
Product Categories/Family for anti-SQSTM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48kDa
NCBI Official Full Name
Sequestosome-1
NCBI Official Synonym Full Names
sequestosome 1
NCBI Official Symbol
SQSTM1
NCBI Official Synonym Symbols
p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
NCBI Protein Information
sequestosome-1
UniProt Protein Name
Sequestosome-1
UniProt Gene Name
SQSTM1
UniProt Synonym Gene Names
ORCA; OSIL; EBIAP; p60
UniProt Entry Name
SQSTM_HUMAN
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Product Notes
The SQSTM1 sqstm1 (Catalog #AAA23550) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SQSTM1 antibody - middle region reacts with Tested Species Reactivity: Human, Mouse Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's SQSTM1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the SQSTM1 sqstm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: EEQMESDNCS GGDDDWTHLS SKEVDPSTGE LQSLQMPESE GPSSLDPSQE. It is sometimes possible for the material contained within the vial of "SQSTM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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