WB (Western Blot)
(Detection of human phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP1 antibody .)
Rabbit anti-Human, Mouse KAP-1 Polyclonal Antibody | anti-KAP-1 antibody
Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified
IP: 6ug/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:200-1:1000
ICC-IF: 1:200-1:1000
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP1 antibody .)
WB (Western Blot)
(Detection of human phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP1 antibody .)
WB (Western Blot)
(Detection of mouse phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3 cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-KAP-1 antibody .)
WB (Western Blot)
(Detection of mouse phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3 cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-KAP-1 antibody .)
IP (Immunoprecipitation)
(Detection of human phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) and rabbit anti-KAP-1 recombinant monoclonal antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), AAA23773 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-KAP1 antibody .)
IP (Immunoprecipitation)
(Detection of human phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) and rabbit anti-KAP-1 recombinant monoclonal antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), AAA23773 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-KAP1 antibody .)
IHC (Immunohistochemistry)
(Detection of human Phospho KAP-1 (S824) by immunohistochemistry. Samples: FFPE serial sections of prostate carcinoma with MOCK CIP treatment (left) and prostate carcinoma with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. AAA23773 lot 6) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Phospho KAP-1 (S824) by immunohistochemistry. Samples: FFPE serial sections of prostate carcinoma with MOCK CIP treatment (left) and prostate carcinoma with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. AAA23773 lot 6) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
ICC (Immunocytochemistry)
(Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. AAA23773) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. AAA23773) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent anti-rabbit IgG-DyLight 594 conjugated used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: FFPE serial sections of asynchronous etoposide treated HeLa cells with MOCK CIP treatment (left) and etoposide treated HeLa cells with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. AAA23773 lot 6) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
ICC (Immunocytochemistry)
(Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: FFPE serial sections of asynchronous etoposide treated HeLa cells with MOCK CIP treatment (left) and etoposide treated HeLa cells with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) (Cat. No. AAA23773 lot 6) used at a dilution of 1:200 (1 ug/ml). Detection: DAB)
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Product Notes
The KAP-1 trim28 (Catalog #AAA23773) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KAP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunocytochemistry-Immunofluorescence (ICC-IF). WB: 1:2000-1:10,000 IP: 6ug/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:200-1:1000 ICC-IF: 1:200-1:1000. Researchers should empirically determine the suitability of the KAP-1 trim28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KAP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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