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WB (Western Blot)
(Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, Jurkat, HDLM-2, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
LAG3 recombinant antibody
Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR028F]
Gene Names
LAG3; CD223
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
LAG3; Monoclonal Recombinant Antibody; Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR028F]; lymphocyte-activation gene 3; Protein FDC; lymphocyte activation gene 3 protein; LAG-3; CD223; CD antigen CD223; Lymphocyte activation gene 3 protein; lymphocyte activating 3; LAG3 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR028F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for LAG3 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Residues 23-450 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, Jurkat, HDLM-2, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, Jurkat, HDLM-2, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used for IP at 20 ul/mg lysate. LAG3 was also immunoprecipitated by a previous lot of this antibody (AAA23802 lot 1) and a second antibody against a different epitope of LAG3 . For blotting immunoprecipitated LAG3, AAA23802 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used for IP at 20 ul/mg lysate. LAG3 was also immunoprecipitated by a previous lot of this antibody (AAA23802 lot 1) and a second antibody against a different epitope of LAG3 . For blotting immunoprecipitated LAG3, AAA23802 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human LAG3 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human LAG3 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human LAG3 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human LAG3 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human LAG3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human LAG3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for LAG3 recombinant antibody
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4 [taken from NCBI Entrez Gene (Gene ID: 3902)]
Product Categories/Family for LAG3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,103 Da
NCBI Official Full Name
lymphocyte activation gene 3 protein
NCBI Official Synonym Full Names
lymphocyte-activation gene 3
NCBI Official Symbol
LAG3
NCBI Official Synonym Symbols
CD223
NCBI Protein Information
lymphocyte activation gene 3 protein
UniProt Protein Name
Lymphocyte activation gene 3 protein
UniProt Gene Name
LAG3
UniProt Synonym Gene Names
FDC; LAG-3
UniProt Entry Name
LAG3_HUMAN
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Product Notes
The LAG3 lag3 (Catalog #AAA23802) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR028F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAG3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the LAG3 lag3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAG3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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