Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us
Cart Login
product-image-AAA23819_WB6.jpg
WB (Western Blot)
>
<
product-image-AAA23819_IHC5.jpg
product-image-AAA23819_IHC4.jpg
product-image-AAA23819_IHC3.jpg
product-image-AAA23819_IHC2.jpg
product-image-AAA23819_ICC.jpg

Nectin-2/CD112 Recombinant Antibody | CD112 recombinant antibody

Rabbit anti-Nectin-2/CD112 Recombinant Monoclonal Antibody [BLR071G]

Gene Names
NECTIN2; HVEB; PRR2; CD112; PVRL2; PVRR2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Purified
Synonyms
Nectin-2/CD112, Recombinant Antibody; Rabbit anti-Nectin-2/CD112 Recombinant Monoclonal Antibody [BLR071G]; CD112; poliovirus receptor-like 2; PVRR2; PVRL2; PRR2; Poliovirus receptor-related protein 2; poliovirus receptor-related 2 (herpesvirus entry mediator B); nectin-2; Nectin cell adhesion molecule 2; HVEB; herpesvirus entry protein B; Herpes virus entry mediator B; CD antigen CD112; Herpesvirus entry mediator B; Nectin-2; nectin cell adhesion molecule 2; CD112 recombinant antibody
Ordering
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR071G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for CD112 recombinant antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF)
Application Notes
WB: 1:1000
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human Nectin-2/CD112 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, Jurkat, U2OS, GaMG, OVCAR-4, 786-O, A-549, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-GAPDH .)

IHC (Immunohistochemistry)

(Detection of human Nectin-2/CD112 in FFPE hepatic carcinoma by IHC-IF. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)

IHC (Immunohistochemistry)

(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)

IHC (Immunohistochemistry)

(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human Nectin-2/CD112 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

Related Product Information for CD112 recombinant antibody
Nectin-2/CD112 is a type I membrane glycoprotein of the Immunoglobulin superfamily. It is a plasma membrane components of adherens junctions and acts as a receptor for certain mutant strains of herpes simplex virus and pseudorabies virus. Nectin-2/CD112 is also a modulator of T-cell signaling. [taken from NCBI Entrez Gene (Gene ID: 389677)].
Product Categories/Family for CD112 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,359 Da
NCBI Official Full Name
nectin-2 isoform alpha
NCBI Official Synonym Full Names
nectin cell adhesion molecule 2
NCBI Official Symbol
NECTIN2
NCBI Official Synonym Symbols
HVEB; PRR2; CD112; PVRL2; PVRR2
NCBI Protein Information
nectin-2
UniProt Protein Name
Nectin-2
UniProt Gene Name
NECTIN2
UniProt Synonym Gene Names
Herpesvirus entry mediator B; HveB

Similar Products

product-image-AAA23819_WB6.jpg

CD112 Recombinant Antibody

Host: Rabbit
Reactivity: Human
Apps: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
product-image-AAA30516_FCM7.jpg

Nectin 2 Antibody

Host: Rabbit
Reactivity: Human, Mouse, Rat
Apps: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
product-image-AAA21321_IHC6.jpg

PVRL4 Antibody

Host: Rabbit
Reactivity: Mouse, Human
Apps: ELISA, Immunohistochemistry, Immunohistochemistry, Western Blot
product-image-AAA13066_SC.jpg

MIP-2 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA21002_SC.jpg

2-AG ELISA Kit

Host: n/a
Reactivity: General
Apps: n/a
product-image-AAA28947_IHC7.jpg

ERK1/2 Antibody

Host: Rabbit
Reactivity: Human, Mouse, Rat
Apps: Western Blot, Immunohistochemistry, Immunofluorescence
product-image-AAA16652_SC.jpg

F1+2 ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA15121_SC.png

GLP-2 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA30746_APP9.png

ERK1/2 Antibody

Host: Rabbit
Reactivity: Human, Mouse, Rat
Apps: Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry, ELISA
product-image-AAA18968_SC.jpg

2-AG ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA11309_SC.jpg

COX-2 ELISA Kit

Host: n/a
Reactivity: Rabbit
Apps: n/a
product-image-AAA13098_SC.jpg

COX-2 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA18993_SC.jpg

ZO-2 ELISA Kit

Host: n/a
Reactivity: Chicken
Apps: n/a
product-image-AAA30003_FCM7.jpg

BCL-2 Antibody

Host: Rabbit
Reactivity: Human, Mouse
Apps: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
product-image-AAA22585_SC.jpg

sTREM-2 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA29001_IHC7.jpg

Cox-2 Antibody

Host: Rabbit
Reactivity: Human
Apps: Western Blot, Immunohistochemistry, Immunofluorescence
product-image-AAA12621_SC.jpg

sVEGFR-2 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA30544_WB7.jpg

Bcl-2 Antibody

Host: Rabbit
Reactivity: Human, Mouse, Rat
Apps: Western Blot, Immunohistochemistry, Immunofluorescence
product-image-AAA23902_APP6.jpg

OCT-2 Antibody

Host: Mouse
Reactivity: Human. Others not known.
Apps: ELISA, Western Blot, Immunohistochemistry

Product Notes

The CD112 nectin2 (Catalog #AAA23819) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Nectin-2/CD112 Recombinant Monoclonal Antibody [BLR071G] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nectin-2/CD112 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF). WB: 1:1000 IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Researchers should empirically determine the suitability of the CD112 nectin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nectin-2/CD112, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.