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WB (Western Blot)
(Detection of human and mouse USP7/HAUSP by western blot. Samples: Whole cell lysate (50 ug) from RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-eEF2 .)
USP7/HAUSP recombinant antibody
Rabbit anti-USP7/HAUSP Recombinant Monoclonal Antibody [BLR072G]
Gene Names
USP7; TEF1; HAUSP
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
USP7/HAUSP; Monoclonal Recombinant Antibody; Rabbit anti-USP7/HAUSP Recombinant Monoclonal Antibody [BLR072G]; TEF1; USP7; ubiquitin-specific-processing protease 7; ubiquitin thioesterase 7; ubiquitin specific protease 7 (herpes virus-associated); deubiquitinating enzyme 7; ubiquitin carboxyl-terminal hydrolase 7; Herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; HAUSP; ubiquitin specific peptidase 7 (herpes virus-associated); Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7; USP7/HAUSP recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR072G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for USP7/HAUSP recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse USP7/HAUSP by western blot. Samples: Whole cell lysate (50 ug) from RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-eEF2 .)
WB (Western Blot)
(Detection of human and mouse USP7/HAUSP by western blot. Samples: Whole cell lysate (50 ug) from RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-eEF2 .)
IP (Immunoprecipitation)
(Detection of human USP7/HAUSP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used for IP at 20 ul/mg lysate. USP7/HAUSP was also immunoprecipitated by rabbit anti-USP7 antibody For blotting immunoprecipitated USP7/HAUSP, AAA23820 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human USP7/HAUSP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used for IP at 20 ul/mg lysate. USP7/HAUSP was also immunoprecipitated by rabbit anti-USP7 antibody For blotting immunoprecipitated USP7/HAUSP, AAA23820 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of mouse USP7/HAUSP by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of mouse USP7/HAUSP by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human USP7/HAUSP by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human USP7/HAUSP by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human USP7/HAUSP by immunocytochemistry. Sample: FFPE section of OVCAR-4 cells. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human USP7/HAUSP by immunocytochemistry. Sample: FFPE section of OVCAR-4 cells. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human USP7/HAUSP (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human USP7/HAUSP (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for USP7/HAUSP recombinant antibody
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 7 (USP7) is a member of this family that has been shown to interact and stabilize p53.
Product Categories/Family for USP7/HAUSP recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
– Da
NCBI Official Full Name
ubiquitin specific protease 7 (herpes virus-associated)
NCBI Official Synonym Full Names
ubiquitin specific peptidase 7 (herpes virus-associated)
NCBI Official Symbol
USP7
NCBI Official Synonym Symbols
TEF1; HAUSP
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 7
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 7
UniProt Gene Name
USP7
UniProt Synonym Gene Names
HAUSP
UniProt Entry Name
UBP7_HUMAN
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Product Notes
The USP7/HAUSP usp7 (Catalog #AAA23820) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP7/HAUSP Recombinant Monoclonal Antibody [BLR072G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's USP7/HAUSP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the USP7/HAUSP usp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP7/HAUSP, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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