CDK1 recombinant antibody

Rabbit anti-CDK1 Recombinant Monoclonal Antibody [BLR085G]

Cat. Num. AAA23826

• Gene Names: CDK1; CDC2; CDC28A; P34CDC2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: CDK1, Recombinant Antibody; Rabbit anti-CDK1 Recombinant Monoclonal Antibody [BLR085G]; cyclin-dependent kinase 1; CDC2; p34 protein kinase; cell division protein kinase 1; cell division cycle 2, G1 to S and G2 to M; cell division control protein 2 homolog; cell cycle controller CDC2; CDK1; CDC28A; P34CDC2; Cyclin-dependent kinase 1; cyclin dependent kinase 1; CDK1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR085G
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for CDK1 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Between 1 and 50
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse CDK1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3 and F9 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin .)

WB (Western Blot)

(Detection of human CDK1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, U2OS, K-562, HEK293T, A-549, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin .)

IP (Immunoprecipitation)

(Detection of human CDK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1) used for IP at 20 ul/mg lysate. CDK1 was also immunoprecipitated by rabbit anti-CDK1 antibodies . For blotting immunoprecipitated CDK1, AAA23826 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human CDK1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human CDK1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human CDK1 in FFPE MOLT4 cells by ICC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human CDK1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CDK1 recombinant monoclonal (AAA23826 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for CDK1 recombinant antibody

Cyclin-dependent kinase 1 (CDK1) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CKD1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control [taken from NCBI Entrez Gene (Gene ID: 983)].

Product Categories/Family for CDK1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502709
• NCBI GeneID: 983
• NCBI Accession #: NP_001777.1
• NCBI GenBank Nucleotide #: NM_001786.4
• UniProt Accession #: P06493; O60764; A8K7C4; C9J497
• Molecular Weight: 34,095 Da
• NCBI Official Full Name: cyclin-dependent kinase 1 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: CDK1
• NCBI Official Synonym Symbols: CDC2; CDC28A; P34CDC2
• NCBI Protein Information: cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
• UniProt Protein Name: Cyclin-dependent kinase 1
• UniProt Gene Name: CDK1
• UniProt Synonym Gene Names: CDC2; CDC28A; CDKN1; P34CDC2; CDK1
• UniProt Entry Name: CDK1_HUMAN