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IP (Immunoprecipitation)
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Rabbit anti-Human CDK1 Polyclonal Antibody | anti-CDK1 antibody

CDK1 Polyclonal Antibody

Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
CDK1, Antibody; CDK1 Polyclonal Antibody; CDK1; CDC2; CDC28A; P34CDC2; cyclin-dependent kinase 1; anti-CDK1 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-CDK1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IP: 50-1:200
Immunogen
A synthetic peptide of human CDK1
Research Area
Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Neuroscience
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.

IP (Immunoprecipitation)

(Immunoprecipitation analysis of 600ug extracts of C6 cells usingug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK1 Polyclonal Antibody at a dilution of 1:1000.)

IP (Immunoprecipitation)

(Immunoprecipitation analysis of 600ug extracts of Jurkat cells usingug CDK1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK1 Polyclonal Antibody at a dilution of 1:1000.)

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using CDK1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts of Jurkat cells using CDK1 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using CDK1 Polyclonal Antibody at 1:1000 dilution.)

Related Product Information for anti-CDK1 antibody
The protein encoded by this gene is member of the Ser/Thr protein kinase family. This protein is catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-CDK1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
983
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,095 Da
NCBI Official Full Name
cyclin-dependent kinase 1 isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 1
NCBI Official Symbol
CDK1
NCBI Official Synonym Symbols
CDC2; CDC28A; P34CDC2
NCBI Protein Information
cyclin-dependent kinase 1; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M
UniProt Protein Name
Cyclin-dependent kinase 1
UniProt Gene Name
CDK1
UniProt Synonym Gene Names
CDC2; CDC28A; CDKN1; P34CDC2; CDK1
UniProt Entry Name
CDK1_HUMAN

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Product Notes

The CDK1 cdk1 (Catalog #AAA22328) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IF: 1:50-1:200 IP: 50-1:200. Researchers should empirically determine the suitability of the CDK1 cdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.