SMAD4 recombinant antibody

Rabbit anti-SMAD4 Recombinant Monoclonal Antibody [BLR133J]

Cat. Num. AAA23844

• Gene Names: SMAD4; JIP; DPC4; MADH4; MYHRS
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: SMAD4; Monoclonal Recombinant Antibody; Rabbit anti-SMAD4 Recombinant Monoclonal Antibody [BLR133J]; mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; SMAD; mothers against DPP homolog 4; SMAD family member 4; MYHRS; Smad4; mothers against decapentaplegic; Drosophila; homolog of; 4; MAD homolog 4; JIP; hSMAD4; DPC4; deletion target in pancreatic carcinoma 4; deleted in pancreatic carcinoma locus 4; SMAD 4; MADH4; Mothers against decapentaplegic homolog 4; SMAD4 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR133J
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for SMAD4 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 6 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
• Immunogen: Between 1 and 50
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse SMAD4 by western blot. Samples: Whole cell lysate (30 ug) from NIH 3T3, CT26, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human SMAD4 by western blot. Samples: Whole cell lysate (1 ug) from HEK293T, GaMG, A-549, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human SMAD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used for IP at 6 ul per reaction. SMAD4 was also immunoprecipitated by rabbit anti-SMAD4 antibody BL-1681B-1A10. For blotting immunoprecipitated SMAD4, AAA23844 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of human SMAD4 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human SMAD4 in FFPE RPMI-8226 cells by immunocytochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human SMAD4 in FFPE Jurkat cells by immunocytochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human Smad4 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Smad4 recombinant monoclonal (AAA23844 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for SMAD4 recombinant antibody

The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD4 forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. SMAD4 functions in the transforming growth factor-beta signaling pathway as well as the bone morphogenetic protein signaling pathway, regulating cell proliferation, angiogenesis, and increasing blood vessel hyperpermeability. [taken from NCBI Entrez Gene (Gene ID: 4089)].

Product Categories/Family for SMAD4 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4885457
• NCBI GeneID: 4089
• NCBI Accession #: NP_005350.1
• NCBI GenBank Nucleotide #: NM_005359.5
• UniProt Accession #: Q13485; A8K405
• Molecular Weight: 60,439 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 4
• NCBI Official Synonym Full Names: SMAD family member 4
• NCBI Official Symbol: SMAD4
• NCBI Official Synonym Symbols: JIP; DPC4; MADH4; MYHRS
• NCBI Protein Information: mothers against decapentaplegic homolog 4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4
• UniProt Protein Name: Mothers against decapentaplegic homolog 4
• UniProt Gene Name: SMAD4
• UniProt Synonym Gene Names: DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
• UniProt Entry Name: SMAD4_HUMAN