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Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using PU.1 Mouse Monoclonal Antibody (PU1/2446). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse PU.1 (SPI-1) Monoclonal Antibody | anti-PU.1 antibody
PU.1 (SPI-1) (B-Cell Marker)
Gene Names
SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
Reactivity
Human. Others not known.
Applications
Western Blot, Immunohistochemistry
Synonyms
PU.1 (SPI-1), Antibody; PU.1 (SPI-1) (B-Cell Marker); Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1; anti-PU.1 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
PU1/2146
Specificity
PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
271
Applicable Applications for anti-PU.1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
WB: 0.5-1ug/ml
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant fragment (around aa 16-170) of human PU.1 protein
Cellular Localization
Nuclear
Hu-chromosome Location
11p11.2
Positive Control
K-562 Cells, THP-1 cells. Lymph Node, Spleen, Hodgkins Lymphoma, Colon Carcinoma.
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using PU.1 Mouse Monoclonal Antibody (PU1/2446). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using PU.1 Mouse Monoclonal Antibody (PU1/2446). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified PU.1 Mouse Monoclonal Antibody (PU1/2146).Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified PU.1 Mouse Monoclonal Antibody (PU1/2146).Confirmation of Integrity and Purity of Antibody.)
WB (Western Blot)
(Western Blot Analysis of THP-1 Cell Lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).)
WB (Western Blot)
(Western Blot Analysis of THP-1 Cell Lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).)
References
Loddenkemper C, et al. J Pathol. 2004; 202:60-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40kDa
NCBI Official Full Name
transcription factor PU.1 isoform 1
NCBI Official Synonym Full Names
Spi-1 proto-oncogene
NCBI Official Symbol
SPI1
NCBI Official Synonym Symbols
OF; PU.1; SFPI1; SPI-1; SPI-A
NCBI Protein Information
transcription factor PU.1
UniProt Protein Name
Transcription factor PU.1
UniProt Gene Name
SPI1
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Product Notes
The PU.1 spi1 (Catalog #AAA23903) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PU.1 (SPI-1) (B-Cell Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's PU.1 (SPI-1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin. WB: 0.5-1ug/ml IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the PU.1 spi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PU.1 (SPI-1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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