Mouse anti-Human HSP60 (Heat Shock Protein 60) Monoclonal Antibody | anti-HSP60 antibody

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Cat. Num. AAA23919

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: HSP60 (Heat Shock Protein 60); Monoclonal Antibody; HSP60 (Heat Shock Protein 60) (Mitochondrial Marker); 60kDa chaperonin; 60kDa heat shock protein mitochondrial; Chaperonin; 60-KD (CPN60); GROEL; HLD4; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (SPG13); anti-HSP60 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CPTC-HSPD1-1
• Specificity: The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes, and the regulation of protein folding. The mitochondrial and cytosolic localization of HSP60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker for T cell recognition, as well as being involved in a danger signal cascade immune response. HSP60 has been shown to influence apoptosis in tumor cells, and changes in its expression level may serve as a biomarker, as down-regulated HSP60 expression indicates a poor prognosis as well as a risk of tumor infiltration development, especially with regard to urothelial carcinomas. In ovarian cancer, decreased expression of HSP60 correlates with aggressive tumor types, while overexpression is correlated with a better patient prognosis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 573

• Applicable Applications for anti-HSP60 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 1-2ug/0.5 million cells in 0.1ml; IF: 1-2ug/ml; WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HEP-2, PC3 or HeLa cells. Breast or Colon Carcinoma.
• Immunogen: Recombinant full-length human HSPD1 protein
• Cellular Localization: Mitochondria in cytoplasm
• Hu-chromosome Location: 2q33.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western Blot Analysis of HEP-2 and PC3 cell lysates using HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells labeling HSP60 with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red))

IF (Immunofluorescence)

(Immunofluorescence Analysis of human MCF-7 cells labeling GPI with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

References

Cheng, Ming Y., et al. 29 Nov. 1990.

NCBI and Uniprot Product Information

• NCBI GI #: 31542947
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NP_002147.2
• UniProt Accession #: P10809
• Molecular Weight: 60kDa
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein family D (Hsp60) member 1
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_HUMAN