Human HSP72 (Heat Shock Protein 72) ELISA Kit | HSP72 elisa kit

Human HSP72 (Heat Shock Protein 72)

Cat. Num. AAA17769

• Reactivity: Human
• Synonyms: HSP72 (Heat Shock Protein 72); Human HSP72 (Heat Shock Protein 72); HSP72 elisa kit

• Reactivity: Human
• Specificity: Specifically recognize HSP72, no obvious cross reaction with other analogues
• Assay Type: Sandwich
• Samples: Cell lysates, tissue homogenates and other biological samples
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for HSP72 elisa kit

Background: Heat Shock Protein 72 (HSP72), also known as Heat Shock Protein 1A (HSPA1A), is a member of the HSP70 family of molecular chaperones. HSP72 promotes the proper folding of nascent polypeptides and assists in the refolding of denatured proteins. However, if either of these processes proceeds too slowly or fails, HSP72 can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein. Pathologically, HSP72 has been implicated in the promotion of multiple cancer types. Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti HSP72 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti HSP72 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with HSP72 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of HSP72 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.