Mouse anti-Human MCM7 Monoclonal Antibody | anti-MCM7 antibody

MCM7 (DNA Replication Licensing Factor MCM7, CDC47 Homolog, P1.1-MCM3, CDC47, MCM2) (Biotin)

Cat. Num. AAA24859

• Gene Names: MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCM7, Antibody; MCM7 (DNA Replication Licensing Factor MCM7, CDC47 Homolog, P1.1-MCM3, CDC47, MCM2) (Biotin); anti-MCM7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6C2
• Specificity: Recognizes human MCM7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MCM7 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-389 from human MCM7 (AAH09398) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Application Data

(Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data

(Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7 HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data

(Detection limit for recombinant GST tagged MCM7 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry)

(Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml])

WB (Western Blot)

(MCM7 monoclonal antibody Western Blot analysis of MCM7 expression in Hela NE.)

WB (Western Blot)

(Western Blot detection against Immunogen (68.53kD).)

Product Categories/Family for anti-MCM7 antibody

Antibodies; DNA Replication; Repair

References

1. Etiological role of human papillomavirus infection for inverted papilloma of the bladder. Shigehara K, Sasagawa T, Doorbar J, Kawaguchi S, Kobori Y, Nakashima T, Shimamura M, Maeda Y, Miyagi T, Kitagawa Y, Kadono Y, Konaka H, Mizokami A, Koh E, Namiki M.J Med Virol. 2011 Feb;83(2):277-85. 2. Etiologic role of human papillomavirus infection in bladder carcinoma. Shigehara K, Sasagawa T, Kawaguchi S, Nakashima T, Shimamura M, Maeda Y, Konaka H, Mizokami A, Koh E, Namiki M.Cancer. 2010 Nov 29. 3. Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion. Lau KM, Chan QK, Pang JC, Li KK, Yeung WW, Chung NY, Lui PC, Tam YS, Li HM, Zhou L, Wang Y, Mao Y, Ng HK.Oncogene. 2010 Jul 26. 4. Validation of different replication markers for the detection of beta-cell proliferation in human pancreatic tissue. Kohler CU, Kreuter A, Rozynkowski MC, Rahmel T, Uhl W, Tannapfel A, Schmidt WE, Meier JJ.Regul Pept. 2010 Jan 14.

NCBI and Uniprot Product Information

• NCBI GI #: 33988389
• NCBI GeneID: 4176
• Molecular Weight: 60,643 Da
• NCBI Official Full Name: Homo sapiens minichromosome maintenance complex component 7, mRNA
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 7
• NCBI Official Symbol: MCM7
• NCBI Official Synonym Symbols: MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• NCBI Protein Information: DNA replication licensing factor MCM7