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Mouse BANF1 Monoclonal Antibody | anti-BANF1 antibody
BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (AP)
Gene Names
BANF1; BAF; NGPS; BCRP1; D14S1460
Applications
Western Blot
Purity
Purified
Synonyms
BANF1, Antibody; BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (AP); Barrier to Autointegration Factor 1; BAF; BCRP1; D14S1460; MGC111161; anti-BANF1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
M2
Specificity
Recognizes BANF1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-BANF1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
BANF1 (AAH05942, 1aa-89aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
WB (Western Blot)
(BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in PC-12.)
WB (Western Blot)
(BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Raw 264.7.)
WB (Western Blot)
(BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Jurkat.)
Application Data
(Detection limit for recombinant GST tagged BANF1 is approximately 10ng/ml as a capture antibody.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-BANF1 antibody
The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.
Product Categories/Family for anti-BANF1 antibody
References
1. Functional Disruption of the Moloney Murine Leukemia Virus Preintegration Complex by Vaccinia-related Kinases. Suzuki Y, Ogawa K, Koyanagi Y, Suzuki Y.J Biol Chem. 2010 Jul 30;285(31):24032-43. Epub 2010 May 28.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,059 Da
NCBI Official Full Name
Barrier to autointegration factor 1
NCBI Official Synonym Full Names
barrier to autointegration factor 1
NCBI Official Symbol
BANF1
NCBI Official Synonym Symbols
BAF; NGPS; BCRP1; D14S1460
NCBI Protein Information
barrier-to-autointegration factor; breakpoint cluster region protein 1
UniProt Protein Name
Barrier-to-autointegration factor
UniProt Gene Name
BANF1
UniProt Synonym Gene Names
BAF; BCRG1
UniProt Entry Name
BAF_HUMAN
