Bovine Tumor necrosis factor ELISA Kit | TNF elisa kit
Bovine Tumor necrosis factor ELISA Kit
Gene Names
TNF; TNFa; TNF-a; TNF-alpha
Reactivity
Bovine
Synonyms
Tumor necrosis factor; N/A; Bovine Tumor necrosis factor ELISA Kit; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF; TNFA; TNFSF2; TNF elisa kit
Reactivity
Bovine
Specificity
Recombinant and natural Bovine Tumor necrosis factor
Sequence Length
234
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.156-10 ng/mL
Sensitivity
< 0.067 ng/mL
Intra-assay Precision
Intra-Assay CV: <=4.9%
Inter-assay Precision
Inter-Assay CV: <=8.1%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Related Product Information for TNF elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,439 Da
NCBI Official Full Name
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
TNFa; TNF-a; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_BOVIN
