Human Myozenin 1 (MYOZ1) ELISA Kit | MYOZ1 elisa kit
Human Myozenin 1 (MYOZ1) ELISA Kit
Gene Names
MYOZ1; CS-2; FATZ; MYOZ
Reactivity
Human
Synonyms
Myozenin 1 (MYOZ1); N/A; Human Myozenin 1 (MYOZ1) ELISA Kit; MYOZ1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MYOZ1. No significant cross-reactivity or interference between MYOZ1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYOZ1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for MYOZ1 elisa kit
Intended Uses: This MYOZ1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MYOZ1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: MYOZ1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MYOZ1 antibody and an MYOZ1-HRP conjugate. The assay sample and buffer are incubated together with MYOZ1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYOZ1 concentration since MYOZ1 from samples and MYOZ1-HRP conjugate compete for the anti-MYOZ1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYOZ1 from the sample, fewer sites are left to bind MYOZ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYOZ1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MYOZ1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MYOZ1 antibody and an MYOZ1-HRP conjugate. The assay sample and buffer are incubated together with MYOZ1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYOZ1 concentration since MYOZ1 from samples and MYOZ1-HRP conjugate compete for the anti-MYOZ1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYOZ1 from the sample, fewer sites are left to bind MYOZ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYOZ1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MYOZ1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,745 Da
NCBI Official Full Name
myozenin-1
NCBI Official Synonym Full Names
myozenin 1
NCBI Official Symbol
MYOZ1
NCBI Official Synonym Symbols
CS-2; FATZ; MYOZ
NCBI Protein Information
myozenin-1; calsarcin-2; filamin-, actinin- and telethonin-binding protein
UniProt Protein Name
Myozenin-1
UniProt Gene Name
MYOZ1
UniProt Synonym Gene Names
MYOZ
UniProt Entry Name
MYOZ1_HUMAN
