Human Anti-Agrin antibody (AGR-Ab) ELISA Kit | AGR-Ab elisa kit

Human Anti-Agrin antibody (AGR-Ab) ELISA Kit

Cat. Num. AAA27382

• Reactivity: Human
• Synonyms: Anti-Agrin antibody (AGR-Ab); N/A; Human Anti-Agrin antibody (AGR-Ab) ELISA Kit; AGR-Ab elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AGRAb. No significant cross-reactivity or interference between AGRAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGRAb and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

Standard Curve (Sample)

Related Product Information for AGR-Ab elisa kit

Principle of the Assay: AGRAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Agrin antigen and an AGRAb-HRP conjugate. The assay sample and buffer are incubated together with AGRAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGRAb concentration since AGRAb from samples and AGRAb-HRP conjugate compete for the Agrin antigen binding site. Since the number of sites is limited, as more sites are occupied by AGRAb from the sample, fewer sites are left to bind AGRAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGRAb concentration in each sample is interpolated from this standard curve.

Intended Uses: This AGRAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGRAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!