Mouse Sphingosine 1-Phosphate ELISA Kit | S1P elisa kit
Mouse Sphingosine 1-Phosphate ELISA Kit
Reactivity
Mouse
Synonyms
Sphingosine 1-Phosphate; N/A; Mouse Sphingosine 1-Phosphate ELISA Kit; S1P elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of SGP1. No significant cross-reactivity or interference between SGP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SGP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Standard Curve (Sample)
Related Product Information for S1P elisa kit
Principle of the Assay: SGP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SGP1 antibody and an SGP1-HRP conjugate. The assay sample and buffer are incubated together with SGP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SGP1 concentration since SGP1 from samples and SGP1-HRP conjugate compete for the anti-SGP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SGP1 from the sample, fewer sites are left to bind SGP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SGP1 concentration in each sample is interpolated from this standard curve.
Intended Uses: This SGP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SGP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This SGP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SGP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for S1P elisa kit
