Mouse anti-Human Histone H3 Monoclonal Antibody | anti-H3 antibody

Anti-Histone H3 Antibody, Mouse Monoclonal

Cat. Num. AAA27711

• Gene Names: HIST1H3A; H3/A; H3FA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A
• Synonyms: Histone H3; Monoclonal Antibody; Anti-Histone H3 Antibody; Mouse Monoclonal; Histone H3 Antibody; Mouse MAb; anti-H3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 1F6D7C4
• Specificity: Human Histone H3
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-H3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-1:10,0000; IHC-P: 1:50-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human Histone H3
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-Histone H3 mouse monoclonal antibody at 1:1000 dilution Lane A: Histone H3 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa)

WB (Western Blot)

(Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution Lysates/proteins at 30 ug per Lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size:18 kDa)

WB (Western Blot)

(Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution Lysates/proteins at 30 ug per Lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size:18 kDa)

WB (Western Blot)

(Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution Lysates/proteins at 30 ug per Lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size:18 kDa)

WB (Western Blot)

(Anti-Histone H3 mouse monoclonal antibody at 1:10000, 1:20000, 1:50000 dilution Lysates/proteins at 30 ug per Lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size:18 kDa)

WB (Western Blot)

(Anti-Histone H3 mouse monoclonal antibody at 1:2000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: 293 Whole Cell Lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/7500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa)

IHC (Immunohistochemistry)

(Immunochemical staining of human histone H3 in human colon carcinoma with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).)

IHC (Immunohistochemistry)

(Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.)

Related Product Information for anti-H3 antibody

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.

References

Bhasin M, et al., 2006, 13(1): 102-12. Lachner M, et al., 2001, 410(6824): 116-20.
Rosenfeld JA, et al., 2009, BMC Genomics. 10: 143.

NCBI and Uniprot Product Information

• NCBI GI #: 4504281
• NCBI GeneID: 8350
• NCBI Accession #: NP_003520.1
• NCBI GenBank Nucleotide #: NM_003529.2
• UniProt Accession #: P68431; P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
• Molecular Weight: 15,404 Da
• NCBI Official Full Name: histone H3.1
• NCBI Official Synonym Full Names: histone cluster 1, H3a
• NCBI Official Symbol: HIST1H3A
• NCBI Official Synonym Symbols: H3/A; H3FA
• NCBI Protein Information: histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
• UniProt Protein Name: Histone H3.1
• UniProt Gene Name: HIST1H3A
• UniProt Synonym Gene Names: H3FA
• UniProt Entry Name: H31_HUMAN