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IP (Immunoprecipitation)
(NES was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-NES mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa Observed band size :177 kDa)
Mouse anti-Human Nestin Monoclonal Antibody | anti-NES antibody
Anti-Nestin Antibody, Mouse Monoclonal
Gene Names
NES; Nbla00170
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Protein A
Synonyms
Nestin; Monoclonal Antibody; Anti-Nestin Antibody; Mouse Monoclonal; Nestin/NES Antibody; Mouse MAb; nestin; Anti-Nbla00170 Antibody; anti-NES antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
11
Specificity
Human Nestin/NES
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NES antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:2000
FC/FACS/FCM: 1:25-1:100
IP: 1-5uL/mg of lysate
IHC-P: 1:200-1:2000
FC/FACS/FCM: 1:25-1:100
IP: 1-5uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Nestin/NES
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the Human Nestin/NES. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(NES was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-NES mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa Observed band size :177 kDa)
IP (Immunoprecipitation)
(NES was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-NES mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa Observed band size :177 kDa)
WB (Western Blot)
(Anti-NES mouse monoclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:177 kDa)
WB (Western Blot)
(Anti-NES mouse monoclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:177 kDa)
FCM (Flow Cytometry)
(Flow cytometric analysis of Human NES expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human NES, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Human NES expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human NES, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
IHC (Immunohistochemistry)
(Immunochemical staining of human Nestin in human fasciculated sarcoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human Nestin in human fasciculated sarcoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human Nestin in human neurilemmoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human Nestin in human neurilemmoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human Nestin in human brain with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human Nestin in human brain with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-NES antibody
NES (Nestin) is a Protein Coding gene. Nestin is a member of the class VI family of intermediate filament proteins, originally identified as a marker of neural stem cells and subsequently demonstrated to be expressed in breast cancer (BC) and other cancer types. Nestin is an intermediate filament protein and participates in the cytoskeleton organization. Nestin expression in the endothelium of blood vessels is mainly limited to newly forming vessels, thus being a more specific marker of angiogenesis than the commonly used vascular antigens. It is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. Nestin is broadly expressed in the heart, fat, and other tissues. Diseases associated with NES include Ependymoblastoma and Periventricular Leukomalacia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1621
NCBI Official Full Name
Nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
Nbla00170
NCBI Protein Information
nestin
UniProt Protein Name
Nestin
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN
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Product Notes
The NES nes (Catalog #AAA27730) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nestin Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:200-1:2000 FC/FACS/FCM: 1:25-1:100 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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