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WB (Western Blot)
(Anti-H2AFM/HIST1H2AB rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: RAW246.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)
Rabbit anti-Human H2AFM/HIST1H2AB Polyclonal Antibody | anti-H2AFM/HIST1H2AB antibody
Anti-H2AFM/HIST1H2AB Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
H2AFM/HIST1H2AB; Polyclonal Antibody; Anti-H2AFM/HIST1H2AB Antibody; Rabbit Polyclonal; histone cluster 1; H2ab; Anti-H2A/m Antibody; Anti-H2AFM Antibody; anti-H2AFM/HIST1H2AB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human H2AFM/HIST1H2AB
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-H2AFM/HIST1H2AB antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
ICC/IF: 1:50-1:200
IHC-P: 1:50-1:200
ICC/IF: 1:50-1:200
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human H2AFM/HIST1H2AB
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human H2AFM/HIST1H2AB, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-H2AFM/HIST1H2AB rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: RAW246.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)
WB (Western Blot)
(Anti-H2AFM/HIST1H2AB rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: RAW246.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human HIST1H2AB in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human HIST1H2AB in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human HIST1H2AB in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human HIST1H2AB in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of Mouse HIST1H2AB in Mouse large intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of Mouse HIST1H2AB in Mouse large intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of Mouse HIST1H2AB in Mouse liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of Mouse HIST1H2AB in Mouse liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IF (Immunofluorescence)
(Immunofluorescence staining of KLH-SMCCA1245 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA1245 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of KLH-SMCCA1245 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA1245 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
Related Product Information for anti-H2AFM/HIST1H2AB antibody
Histone H2A type 1-B/E is a protein that in humans is encoded by the H2AC4 (H2A Clustered Histone 4, also known as HIST1H2AB) gene. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3, and an important paralog of this gene is H2AC8. H2AC4 is a core component of nucleosome. Diseases associated with H2AC4 include Alpha-Thalassemia/Mental Retardation Syndrome, X-Linked. Among its related pathways are Chromatin organization and Signaling by GPCR.
References
Zhang S, et al. (2019) Cadmium disrupts the DNA damage response by destabilizing rnf168. Food Chem Toxicol 133 110745.
Sawasdikosol S, et al. (2007) Prostaglandin e2 activates hpk1 kinase activity via a pka-dependent pathway. J Biol Chem 282 (48): 34693-34699.
Ai H, et al. (2019) Examination of the deubiquitylation site selectivity of usp51 by using chemically synthesized ubiquitylated histones. Chembiochem 20 (2): 221-229.
Sawasdikosol S, et al. (2007) Prostaglandin e2 activates hpk1 kinase activity via a pka-dependent pathway. J Biol Chem 282 (48): 34693-34699.
Ai H, et al. (2019) Examination of the deubiquitylation site selectivity of usp51 by using chemically synthesized ubiquitylated histones. Chembiochem 20 (2): 221-229.
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Product Notes
The H2AFM/HIST1H2AB (Catalog #AAA27772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-H2AFM/HIST1H2AB Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's H2AFM/HIST1H2AB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC-P: 1:50-1:200 ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the H2AFM/HIST1H2AB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H2AFM/HIST1H2AB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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