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Application Data
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Mouse Cytokeratin 14 Monoclonal Antibody | anti-CK14 antibody

Cytokeratin 14 (ABT047) Antibody

Gene Names
KRT14; K14; NFJ; CK14; EBS3; EBS4
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, ELISA
Purity
Protein G
Synonyms
Cytokeratin 14, Antibody; Cytokeratin 14 (ABT047) Antibody; Cytokeratin-14; Keratin, type I cytoskeletal 14 (Cytokeratin-14; CK-14; Keratin-14; K14); anti-CK14 antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Mouse IgG2b, kappa
Clone Number
ABT047
Specificity
The antibody can specifically recognize human CK14 protein. In immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody specifically labels the basal cell of squamous epithelial cells and glandular epithelia, myoepithelium and mesothelial cells. In western blotting of A431 cell lysate, the antibody can label a 50 kDa band.
Purity/Purification
Protein G
Form/Format
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Applicable Applications for anti-CK14 antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), ELISA
Application Notes
IHC: 1:200-1000
WB: 1:500-2000
IF: 1:100-500
ELISA: 1:1000-5000
Preparation and Storage
Store at -20 degree C/1 year

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SDS-PAGE

(Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK14 (ABT047) antibody. The HRP-conjugated Goat anti-M)

Product Categories/Family for anti-CK14 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
472
NCBI Official Full Name
Keratin, type I cytoskeletal 14
NCBI Official Synonym Full Names
keratin 14
NCBI Official Symbol
KRT14
NCBI Official Synonym Symbols
K14; NFJ; CK14; EBS3; EBS4
NCBI Protein Information
keratin, type I cytoskeletal 14; CK-14; keratin-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
UniProt Protein Name
Keratin, type I cytoskeletal 14
UniProt Gene Name
KRT14
UniProt Synonym Gene Names
CK-14; K14
UniProt Entry Name
K1C14_HUMAN

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Product Notes

The CK14 krt14 (Catalog #AAA30881) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 14 (ABT047) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 14 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), ELISA. IHC: 1:200-1000 WB: 1:500-2000 IF: 1:100-500 ELISA: 1:1000-5000. Researchers should empirically determine the suitability of the CK14 krt14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.