Standard Curve (Sample)
General Catalase Assay Kit | CAT assay kit
Catalase Microplate Assay Kit
Reactivity
General
Applications
Functional Assay
Synonyms
Catalase; Catalase Microplate Assay Kit; EC 1.11.1.6; CAT assay kit
Reactivity
General
Sequence Length
527
Samples
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples
Detection Range
0.5 mmol/L - 50 mmol/L
Applicable Applications for CAT assay kit
Functional Assay
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CAT assay kit
Background: Catalase is an antioxidant enzyme ubiquitously present in mammalian and non-mammalian aerobic cells containing a cytochrome system. It was initially isolated from ox liver and later from blood, bacterial, and plant sources. The enzyme contains 4 ferrihemoprotein groups per molecule. The enzyme has a molecular mass of 240 kDa. Catalase activity varies greatly between tissues. The activity is highest in the liver and kidney, and lowest in connective tissues. In eukaryotic cells the enzyme is concentrated in the subcellular organelles called peroxisomes (microbodies). Catalase catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidase and superoxide dismutase reactions. Hydrogen peroxide is highly deleterious to the cell and its accumulation causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to the cell. It's role in oxidative stress related diseases has been widely studied. The assay is initiated with the enzymatic hydrolysis of H2O2 by CAT. The reaction product can be react with the dry reagent, and measured at a colorimetric readout at 405 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,756 Da
NCBI Official Full Name
catalase
NCBI Official Synonym Full Names
catalase
NCBI Official Symbol
CAT
NCBI Protein Information
catalase
UniProt Protein Name
Catalase
UniProt Gene Name
CAT
UniProt Entry Name
CATA_HUMAN
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Product Notes
The General CAT cat (Catalog #AAA27850) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27850 Assay Kit recognizes General CAT. AAA Biotech's Catalase can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the CAT cat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Catalase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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