ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR? antibody (AAA28486) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Rabbit anti-Human RXRalpha Monoclonal Antibody | anti-RXRA antibody
RXRalpha Rabbit mAb
IF/ICC: 1:100-1:1000
IP: 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
ELISA: Recommended starting concentration is 1ug/mL.
Please optimize the concentration based on your specific assay requirements.
ChIP: 5ug antibody for 10ug-15ug of Chromatin
ChIP-seq: 1:50-1:100
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR? antibody (AAA28486) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR? antibody (AAA28486) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts from HepG2 cells using 3 ug RXR? antibody (AAA28486). Western blot was performed from the immunoprecipitate using RXR? antibody (AAA28486) at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts from HepG2 cells using 3 ug RXR? antibody (AAA28486). Western blot was performed from the immunoprecipitate using RXR? antibody (AAA28486) at a dilution of 1:1000.)
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using RXR? Rabbit mAb (AAA28486,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using RXR? Rabbit mAb (AAA28486,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)
WB (Western Blot)
(Western blot analysis of various lysates using RXR? Rabbit mAb (AAA28486) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of various lysates using RXR? Rabbit mAb (AAA28486) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of various lysates using RXR? Rabbit mAb (AAA28486) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)
WB (Western Blot)
(Western blot analysis of various lysates using RXR? Rabbit mAb (AAA28486) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR? Rabbit mAb (AAA28486). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR? in the representative genomic region surrounding ECH1 gene.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR? Rabbit mAb (AAA28486). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR? in the representative genomic region surrounding ECH1 gene.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR? Rabbit mAb (AAA28486). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR? across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR? (lower panel).)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR? Rabbit mAb (AAA28486). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR? across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR? (lower panel).)
NCBI and Uniprot Product Information
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Product Notes
The RXRA rxra (Catalog #AAA28486) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RXRalpha Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RXRalpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation (ChIP)-seq. WB: 1:1000-1:2000 IF/ICC: 1:100-1:1000 IP: 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells ELISA: Recommended starting concentration is 1ug/mL. Please optimize the concentration based on your specific assay requirements. ChIP: 5ug antibody for 10ug-15ug of Chromatin ChIP-seq: 1:50-1:100. Researchers should empirically determine the suitability of the RXRA rxra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDTKHFLPLD FSTQVNSSLT SPTGRGSMAA PSLHPSLGPG IGSPGQLHSP ISTLSSPING MGPPFSVISS PMGPHSMSVP TTPTLGFSTG SPQLSSPMNP. It is sometimes possible for the material contained within the vial of "RXRalpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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