Rabbit anti-Human STAT3-Y705 Monoclonal Antibody | anti-STAT3 antibody

Phospho-STAT3-Y705 Rabbit mAb

Cat. Num. AAA28610

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity purification
• Synonyms: STAT3-Y705, Antibody; Phospho-STAT3-Y705 Rabbit mAb; APRF; HIES; ADMIO; ADMIO1; Phospho-STAT3-Y705; anti-STAT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: APYLK

• Applicable Applications for anti-STAT3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:50-1:200; IF/ICC: 1:50-1:200; ELISA: Recommended starting concentration is 1ug/mL.; Please optimize the concentration based on your specific assay requirements.
• Cross Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic phosphorylated peptide around Y705 of human STAT3 (NP_644805.1).
• Modification: Phospho Y705
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

ICC (Immunocytochemistry)

(Confocal imaging of paraffin-embedded Mouse colon tissue using Phospho-STAT3-Y705 Rabbit mAb (AAA28610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistchemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-? (20 ng/mL) at 37? for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

Related Product Information for anti-STAT3 antibody

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.

Product Categories/Family for anti-STAT3 antibody

Epigenetics Nuclear Signaling; Transcription Factors; Protein phosphorylation; Cancer; Signal Transduction; ErbB-HER Signaling Pathway; MAPK-Erk Signaling Pathway; MAPK-JNK Signaling Pathway; Cell Biology Developmental Biology; Apoptosis; Inhibition of Ap

NCBI and Uniprot Product Information

• NCBI GI #: 21618338
• NCBI GeneID: 6774
• NCBI Accession #: NP_003141.2
• NCBI GenBank Nucleotide #: NM_003150.3
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8
• Molecular Weight: Calculated MW: 88kDa; Observed MW: 79kDa/88kDa
• UniProt Protein Name: Signal transducer and activator of transcription 3
• UniProt Gene Name: STAT3
• UniProt Synonym Gene Names: APRF
• UniProt Entry Name: STAT3_HUMAN