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WB (Western Blot) (Detection of human STAT3 by western blot. Samples: Whole cell lysate (50 ug) from RKO, NCI-H226, HEK293T, GaMG, LNCaP, HeLa, Ramos, MOLT-4, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

STAT3 recombinant antibody

Rabbit anti-STAT3 Recombinant Monoclonal Antibody [BLR098G]

Gene Names
STAT3; APRF; HIES
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
STAT3; Monoclonal Recombinant Antibody; Rabbit anti-STAT3 Recombinant Monoclonal Antibody [BLR098G]; acute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; HIES; signal transducer and activator of transcription 3; Signal transducer and activator of transcription 3; STAT3 recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR098G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for STAT3 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human STAT3 by western blot. Samples: Whole cell lysate (50 ug) from RKO, NCI-H226, HEK293T, GaMG, LNCaP, HeLa, Ramos, MOLT-4, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of human STAT3 by western blot. Samples: Whole cell lysate (50 ug) from RKO, NCI-H226, HEK293T, GaMG, LNCaP, HeLa, Ramos, MOLT-4, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of human STAT3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H226 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1) used for IP at 6 ul per reaction. STAT3 was also immunoprecipitated by rabbit anti-STAT3 antibody For blotting immunoprecipitated STAT3, AAA23832 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of human STAT3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H226 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1) used for IP at 6 ul per reaction. STAT3 was also immunoprecipitated by rabbit anti-STAT3 antibody For blotting immunoprecipitated STAT3, AAA23832 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemistry)

(Detection of human STAT3 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human STAT3 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of mouse STAT3 in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of mouse STAT3 in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human STAT3 in FFPE H226 cells by ICC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human STAT3 in FFPE H226 cells by ICC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human STAT3 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-STAT3 recombinant monoclonal (AAA23832) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human STAT3 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-STAT3 recombinant monoclonal (AAA23832) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for STAT3 recombinant antibody
STAT3 (signal transducer and activator of transcription 3) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein [taken from NCBI Entrez Gene (GeneID:6774)].
Product Categories/Family for STAT3 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,068 Da
NCBI Official Full Name
signal transducer and activator of transcription 3 isoform 1
NCBI Official Synonym Full Names
signal transducer and activator of transcription 3 (acute-phase response factor)
NCBI Official Symbol
STAT3
NCBI Official Synonym Symbols
APRF; HIES
NCBI Protein Information
signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
UniProt Protein Name
Signal transducer and activator of transcription 3
UniProt Gene Name
STAT3
UniProt Synonym Gene Names
APRF
UniProt Entry Name
STAT3_HUMAN

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Product Notes

The STAT3 stat3 (Catalog #AAA23832) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-STAT3 Recombinant Monoclonal Antibody [BLR098G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STAT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the STAT3 stat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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