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WB (Western Blot) (Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78/74 kD Observed band size: 78/74 kD)

Rabbit MARCH7 Polyclonal Antibody | anti-MARCH7 antibody

MARCH7 Polyclonal Antibody

Gene Names
MARCH7; AXO; AXOT; RNF177; MARCH-VII
Reactivity
Rat, Pig, Dog, Cow
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MARCH7; Polyclonal Antibody; MARCH7 Polyclonal Antibody; E3 ubiquitin-protein ligase MARCHF7; 2.3.2.27; Axotrophin; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; MARCH-VII; RING finger protein 177; RING-type E3 ubiquitin transferase MARCHF7; MARCHF7; AXOT; RNF177; anti-MARCH7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-MARCH7 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78/74 kD Observed band size: 78/74 kD)

WB (Western Blot) (Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78/74 kD Observed band size: 78/74 kD)

WB (Western Blot)

(Sample: Siha(human)cell Lysate at 30 ug MCF-7(human)cell Lysate at 30 ug U251(human)cell Lysate at 30 ug testis(rat) Lysate at 40 ug Primary: Anti-MARCH7 (bs-9341R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78kD Observed band size: 73 kD)

WB (Western Blot) (Sample: Siha(human)cell Lysate at 30 ug MCF-7(human)cell Lysate at 30 ug U251(human)cell Lysate at 30 ug testis(rat) Lysate at 40 ug Primary: Anti-MARCH7 (bs-9341R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78kD Observed band size: 73 kD)

IHC (Immunohistochemistry-Paraffin)

(Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4 degree C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.)

IHC (Immunohistochemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4 degree C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.)

IHC (Immunohistochemistry-Paraffin)

(Dilution: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4 degree C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4 degree C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.)

WB (Western Blot)

(Sample: Siha(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD)

WB (Western Blot) (Sample: Siha(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD)

WB (Western Blot)

(Sample: Siha(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD)

WB (Western Blot) (Sample: Siha(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,778 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH7 isoform a
NCBI Official Synonym Full Names
membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase
NCBI Official Symbol
MARCH7
NCBI Official Synonym Symbols
AXO; AXOT; RNF177; MARCH-VII
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH7; axotrophin; RING finger protein 177; membrane-associated RING-CH protein VII; membrane-associated RING finger protein 7
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH7
UniProt Gene Name
MARCH7
UniProt Synonym Gene Names
AXOT; RNF177; MARCH-VII
UniProt Entry Name
MARH7_HUMAN

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Product Notes

The MARCH7 march7 (Catalog #AAA28826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCH7 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MARCH7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF). Researchers should empirically determine the suitability of the MARCH7 march7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCH7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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