Mouse anti-Human, Mouse PCNA Monoclonal Antibody | anti-PCNA antibody

PCNA Mouse Monoclonal Antibody

Cat. Num. AAA29641

• Gene Names: PCNA; ATLD2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification using immunogen.
• Synonyms: PCNA; Monoclonal Antibody; PCNA Mouse Monoclonal Antibody; anti-PCNA antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Specificity: Antibody can detect endogenous?PCNA protein.
• Purity/Purification: Affinity purification using immunogen.
• Form/Format: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-PCNA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:5000; Immunohistochemistry: 1:200
• Target Name: PCNA
• Preparation and Storage: Store at -20 degree C

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, PCNA Monoclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 wasused for antibody retrieval(>98C,20min). 3,Secondaryantibody was diluted at 1:200(room tempeRature, 30min).Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, PCNA Monoclonal Antibody was diluted at 1:200 (4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, PCNA Monoclonal Antibody was diluted at 1:200 (4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min).Negative control was used by secondary antibody only.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Rat-testis tissue. 1,PCNA Monoclonal Antibody (red) was diluted at 1:200 (4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. PictureC: merge of A+B.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Human-lung-cancer tissue. 1,PCNA Monoclonal Antibody(red) was diluted at 1:200 (4C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.)

IHC (Immunohistochemistry)

(IHC staining of paraffin- embedded Human breast cancer tissue with PCNA mouse mAb (12D10) diluted at 1:200.)

WB (Western Blot)

(Western blot analysis of Hela (lane 1), Rat brain (lane 2), NIH 3T3 (lane 3), 293T (lane 4), using diluted at 1:5000.)

Related Product Information for anti-PCNA antibody

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Product Categories/Family for anti-PCNA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 49456555
• NCBI GeneID: 5111
• UniProt Accession #: Q6FHF5; P12004
• Molecular Weight: SDS-PAGE MW: 36kd
• NCBI Official Full Name: PCNA, partial
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen
• UniProt Protein Name: Proliferating cell nuclear antigen
• UniProt Gene Name: PCNA
• UniProt Entry Name: Q6FHF5_HUMAN