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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Mouse anti-Human PBK Monoclonal Antibody | anti-PBK antibody
PBK Antibody
Gene Names
PBK; SPK; CT84; TOPK; Nori-3
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PBK; Monoclonal Antibody; PBK Antibody; Cancer/testis antigen 84 antibody; CT84 antibody; Epididymis luminal protein 164 antibody; FLJ14385 antibody; HEL164 antibody; Lymphokine activated killer T cell originated protein kinase antibody; Lymphokine-activated killer T-cell-originated protein kinase antibody; MAPKK like protein kinase antibody; MAPKK-like protein kinase antibody; Nori 3 antibody; Nori-3 antibody; Nori3 antibody; PBK antibody; PDZ binding kinase antibody; PDZ-binding kinase antibody; Serine/threonine protein kinase antibody; Spermatogenesis related protein kinase antibody; Spermatogenesis-related protein kinase antibody; SPK antibody; T LAK cell originated protein kinase antibody; T-LAK cell-originated protein kinase antibody; TOPK antibody; TOPK_HUMAN antibody; anti-PBK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
E11-C8
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PBK antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
IHC: 1:100-1:500
ICC: 1:100-1:500
FC/FACS: 1:100-1:200
IHC: 1:100-1:500
ICC: 1:100-1:500
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry)
(ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1, 000 dilution.)
Related Product Information for anti-PBK antibody
Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states. Human PDZ-binding kinase, known as PBK, is a 322 amino acid, T/SXV motif-containing serine/threonine kinase that is abuntant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. The cell cycle checkpoint kinase Cdc2/cyclin B is an upstream effector of PBK that can phosphorylate and activate PBK. Active PBK may associate with PDZ-containing proteins and influence cell cycle control or cellular proliferation.
Product Categories/Family for anti-PBK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,085 Da
NCBI Official Full Name
lymphokine-activated killer T-cell-originated protein kinase
NCBI Official Synonym Full Names
PDZ binding kinase
NCBI Official Symbol
PBK
NCBI Official Synonym Symbols
SPK; CT84; TOPK; Nori-3
NCBI Protein Information
lymphokine-activated killer T-cell-originated protein kinase; PDZ-binding kinase; cancer/testis antigen 84; MAPKK-like protein kinase; serine/threonine protein kinase; T-LAK cell-originated protein kinase; spermatogenesis-related protein kinase
UniProt Protein Name
Lymphokine-activated killer T-cell-originated protein kinase
UniProt Gene Name
PBK
UniProt Synonym Gene Names
TOPK; CT84; SPK
UniProt Entry Name
TOPK_HUMAN
Similar Products
Product Notes
The PBK pbk (Catalog #AAA29912) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PBK Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PBK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:100-1:500 ICC: 1:100-1:500 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the PBK pbk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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