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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Glut1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Rabbit anti-Human, Mouse Glut-1 Polyclonal Antibody | anti-Glut-1 antibody

Glut-1 Antibody

Gene Names
SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT1DS
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide affinity purified
Synonyms
Glut-1; Polyclonal Antibody; Glut-1 Antibody; Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody; CSE antibody; DYT17 antibody; DYT18 antibody; DYT9 antibody; EIG12 antibody; erythrocyte/brain antibody; Erythrocyte/hepatoma glucose transporter antibody; facilitated glucose transporter member 1 antibody; Glucose transporter 1 antibody; Glucose transporter type 1 antibody; Glucose transporter type 1; GLUT antibody; GLUT-1 antibody; GLUT1 antibody; GLUT1DS antibody; GLUTB antibody; GT1 antibody; GTG1 antibody; Gtg3 antibody; GTR1_HUMAN antibody; HepG2 glucose transporter antibody; HTLVR antibody; Human T cell leukemia virus (I and II) receptor antibody; MGC141895 antibody; MGC141896 antibody; PED antibody; RATGTG1 antibody; Receptor for HTLV 1 and HTLV 2 antibody; SLC2A1 antibody; Solute carrier family 2 (facilitated glucose transporter); member 1 antibody; Solute carrier family 2 antibody; Solute carrier family 2; anti-Glut-1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Glut-1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Immunogen
Peptide.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with Glut1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Glut1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining Glut1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Glut1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Glut1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Glut1 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Glut1 on human placenta tissue lysates using anti-Glut1 antibody at 1/1, 000 dilution.)

WB (Western Blot) (Western blot analysis of Glut1 on human placenta tissue lysates using anti-Glut1 antibody at 1/1, 000 dilution.)
Related Product Information for anti-Glut-1 antibody
Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane, and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve-pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.
Product Categories/Family for anti-Glut-1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,084 Da
NCBI Official Full Name
solute carrier family 2, facilitated glucose transporter member 1
NCBI Official Synonym Full Names
solute carrier family 2 (facilitated glucose transporter), member 1
NCBI Official Symbol
SLC2A1
NCBI Official Synonym Symbols
PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT1DS
NCBI Protein Information
solute carrier family 2, facilitated glucose transporter member 1; GLUT-1; hepG2 glucose transporter; glucose transporter type 1, erythrocyte/brain; human T-cell leukemia virus (I and II) receptor
UniProt Protein Name
Solute carrier family 2, facilitated glucose transporter member 1
UniProt Gene Name
SLC2A1
UniProt Synonym Gene Names
GLUT1; GLUT-1
UniProt Entry Name
GTR1_HUMAN

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Product Notes

The Glut-1 slc2a1 (Catalog #AAA29922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glut-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glut-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the Glut-1 slc2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glut-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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