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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Rabbit anti-Human, Mouse ApoER2 Polyclonal Antibody | anti-ApoER2 antibody

ApoER2 Antibody

Gene Names
LRP8; MCI1; LRP-8; APOER2; HSZ75190
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Protein affinity purified
Synonyms
ApoER2; Polyclonal Antibody; ApoER2 Antibody; APOER2 antibody; Apolipoprotein E receptor 2 antibody; low density lipoprotein receptor-related protein 8 antibody; Low-density lipoprotein receptor-related protein 8 antibody; LRP-8 antibody; LRP8 antibody; LRP8_HUMAN antibody; anti-ApoER2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Protein affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ApoER2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50
ICC: 1:50-1:200
FC: 1:50-1:100
Immunogen
Recombinant protein within human ApoER2 500-750 aa.
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining ApoER2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ApoER2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining ApoER2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ApoER2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining ApoER2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ApoER2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ApoER2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ApoER2 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1, 000 dilution. Positive control Ω 1/2 Lane1: Mouse placenta Lane2: SH-SY-5Y)

WB (Western Blot) (Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1, 000 dilution. Positive control Ω 1/2 Lane1: Mouse placenta Lane2: SH-SY-5Y)
Related Product Information for anti-ApoER2 antibody
ApoER2 (apolipoprotein E receptor 2), also designated LRP8, is a member of the LDL receptor gene family, which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. ApoER2 is expressed in brain and placenta and has several splice variants. ApoER2 is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.
Product Categories/Family for anti-ApoER2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77 kDa
NCBI Official Full Name
low-density lipoprotein receptor-related protein 8 isoform 4
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
NCBI Official Symbol
LRP8
NCBI Official Synonym Symbols
MCI1; LRP-8; APOER2; HSZ75190
NCBI Protein Information
low-density lipoprotein receptor-related protein 8; ApoE receptor 2
UniProt Protein Name
Low-density lipoprotein receptor-related protein 8
UniProt Gene Name
LRP8
UniProt Synonym Gene Names
APOER2; LRP-8
UniProt Entry Name
LRP8_HUMAN

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Product Notes

The ApoER2 lrp8 (Catalog #AAA29943) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ApoER2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ApoER2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC). WB: 1:500-1:1000 IHC: 1:50 ICC: 1:50-1:200 FC: 1:50-1:100. Researchers should empirically determine the suitability of the ApoER2 lrp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ApoER2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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