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ICC (Immunocytochemistry)
(ICC staining CPT2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit CPT2 Monoclonal Antibody | anti-CPT2 antibody
CPT2 Antibody
Gene Names
CPT2; CPT1; IIAE4; CPTASE
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
CPT2; Monoclonal Antibody; CPT2 Antibody; Carnitine O palmitoyltransferase 2 antibody; Carnitine O palmitoyltransferase 2 mitochondrial antibody; Carnitine O-palmitoyltransferase 2 antibody; Carnitine palmitoyltransferase 2 antibody; Carnitine palmitoyltransferase II antibody; CPT 1 antibody; CPT 2 antibody; CPT II antibody; CPT1 antibody; CPT2 antibody; CPT2_HUMAN antibody; CPTASE antibody; CPTII antibody; IIAE4 antibody; mitochondrial antibody; anti-CPT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SN06-70
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CPT2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining CPT2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CPT2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CPT2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining CPT2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CPT2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CPT2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of CPT2 on different lysates using anti-CPT2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: HepG2 Lane 4: NIH/3T3)
WB (Western Blot)
(Western blot analysis of CPT2 on different lysates using anti-CPT2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: HepG2 Lane 4: NIH/3T3)
Related Product Information for anti-CPT2 antibody
The mitochondrial beta-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.
Product Categories/Family for anti-CPT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
658
NCBI Official Full Name
Carnitine O-palmitoyltransferase 2, mitochondrial
NCBI Official Synonym Full Names
carnitine palmitoyltransferase 2
NCBI Official Symbol
CPT2
NCBI Official Synonym Symbols
CPT1; IIAE4; CPTASE
NCBI Protein Information
carnitine O-palmitoyltransferase 2, mitochondrial; CPT II; carnitine palmitoyltransferase II
UniProt Protein Name
Carnitine O-palmitoyltransferase 2, mitochondrial
UniProt Gene Name
CPT2
UniProt Synonym Gene Names
CPT1; CPT II
UniProt Entry Name
CPT2_HUMAN
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Product Notes
The CPT2 cpt2 (Catalog #AAA30173) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CPT2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CPT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CPT2 cpt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPT2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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