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IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.)

Rabbit S100A4 Monoclonal Antibody | anti-S100A4 antibody

S100A4 Antibody

Gene Names
S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity-chromatography
Synonyms
S100A4; Monoclonal Antibody; S100A4 Antibody; 18A2 antibody; 42A antibody; calcium Placental protein antibody; Calvasculin antibody; CAPL antibody; Fibroblast specific protein 1 (FSP1) antibody; Fibroblast specific protein 1 antibody; Fibroblast specific protein antibody; FSP1 antibody; Leukemia multidrug resistance associated protein antibody; Malignant transformation suppression 1 (MTS1) antibody; Malignant transformation suppression 1 antibody; Metastasin antibody; MTS1 antibody; OTTHUMP00000015467 antibody; OTTHUMP00000015468 antibody; P9KA antibody; PEL98 antibody; Placental calcium-binding protein antibody; Protein Mts1 antibody; Protein S100 A4 antibody; Protein S100-A4 antibody; S100 calcium binding protein A4 (calcium protein; calvasculin; metastasin; murine placental homolog) antibody; S100 calcium binding protein A4 antibody; S100 calcium-binding protein A4 antibody; S100a4 antibody; S10A4_HUMAN antibody; anti-S100A4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SD200-08
Purity/Purification
Affinity-chromatography
Form/Format
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Concentration
1.2mg/ml (varies by lot)
Applicable Applications for anti-S100A4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:100-1:200
ICC/IF: 1:50-1:200
IP: 1:20-1:50
FC: 1:20-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Preparation and Storage
Store at 4 degree C for short term.
Store at -20 degree C for long term.
Avoid freeze/thaw cycle

IF (Immunofluorescence)

(Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.)

IF (Immunofluorescence) (Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S100A4 Antibody)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S100A4 Antibody)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 500 dilution.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 500 dilution.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:200 dilution.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:200 dilution.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:200 dilution.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:200 dilution.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.)

WB (Western Blot)

(Western blot analysis of S100A4 expression in A375 cell lysate.)

WB (Western Blot) (Western blot analysis of S100A4 expression in A375 cell lysate.)

WB (Western Blot)

(All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.)

FCM (Flow Cytometry)

(Flow cytometric analysis of Jurkat cells with S100A4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with S100A4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining S100A4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining S100A4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining S100A4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining S100A4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining S100A4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining S100A4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-S100A4 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-S100A4 antibody
Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy.
Product Categories/Family for anti-S100A4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
12 kDa
NCBI Official Full Name
Protein S100-A4
NCBI Official Synonym Full Names
S100 calcium binding protein A4
NCBI Official Symbol
S100A4
NCBI Official Synonym Symbols
42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
NCBI Protein Information
protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metas
UniProt Protein Name
Protein S100-A4
UniProt Gene Name
S100A4
UniProt Synonym Gene Names
CAPL; MTS1
UniProt Entry Name
S10A4_HUMAN

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Product Notes

The S100A4 s100a4 (Catalog #AAA30187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S100A4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S100A4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:100-1:200 ICC/IF: 1:50-1:200 IP: 1:20-1:50 FC: 1:20-1:100. Researchers should empirically determine the suitability of the S100A4 s100a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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