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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Peroxiredoxin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit Peroxiredoxin 2 Monoclonal Antibody | anti-PRDX2 antibody
Peroxiredoxin 2 Antibody
Gene Names
PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Peroxiredoxin 2; Monoclonal Antibody; Peroxiredoxin 2 Antibody; Epididymis secretory sperm binding protein Li 2a antibody; HEL S 2a antibody; MGC4104 antibody; Natural killer cell enhancing factor B antibody; Natural killer cell-enhancing factor B antibody; Natural Killer Enhancing Factor B antibody; NKEF B antibody; NKEF-B antibody; NKEFB antibody; Peroxiredoxin-2 antibody; PRDX 2 antibody; PRDX2 antibody; PRDX2_HUMAN antibody; PrP antibody; PRX2 antibody; PRXII antibody; PTX1 antibody; TDPX1 antibody; Thiol Specific Antioxidant 1 antibody; Thiol specific antioxidant protein antibody; Thiol-specific antioxidant protein antibody; Thioredoxin Dependent Peroxide Reductase 1 antibody; Thioredoxin peroxidase 1 antibody; Thioredoxin-dependent peroxide reductase 1 antibody; Torin antibody; TPX1 antibody; TSA antibody; anti-PRDX2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JJ090-3
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PRDX2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:10-1:50
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:10-1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Peroxiredoxin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Peroxiredoxin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Peroxiredoxin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Peroxiredoxin 2 on different lysates using anti-Peroxiredoxin 2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2)
WB (Western Blot)
(Western blot analysis of Peroxiredoxin 2 on different lysates using anti-Peroxiredoxin 2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2)
Related Product Information for anti-PRDX2 antibody
The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.
Product Categories/Family for anti-PRDX2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,892 Da
NCBI Official Full Name
PRDX2
NCBI Official Synonym Full Names
peroxiredoxin 2
NCBI Official Symbol
PRDX2
NCBI Official Synonym Symbols
PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B
NCBI Protein Information
peroxiredoxin-2; torin; thioredoxin peroxidase 1; thiol-specific antioxidant 1; natural killer cell-enhancing factor B; thioredoxin-dependent peroxide reductase 1
UniProt Protein Name
Peroxiredoxin-2
UniProt Gene Name
PRDX2
UniProt Synonym Gene Names
NKEFB; TDPX1; NKEF-B; TSA
UniProt Entry Name
PRDX2_HUMAN
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Product Notes
The PRDX2 prdx2 (Catalog #AAA30240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Peroxiredoxin 2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxiredoxin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:10-1:50. Researchers should empirically determine the suitability of the PRDX2 prdx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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