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FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with SATB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Rabbit SATB2 Monoclonal Antibody | anti-SATB2 antibody
SATB2 Antibody
Gene Names
SATB2; GLSS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
SATB2; Monoclonal Antibody; SATB2 Antibody; DNA binding protein SATB2 antibody; DNA-binding protein SATB2 antibody; FLJ21474 antibody; FLJ32076 antibody; GLSS antibody; KIAA1034 antibody; MGC119474 antibody; MGC119477 antibody; SATB family member 2 antibody; SATB homeobox 2 antibody; SATB2 antibody; SATB2_HUMAN antibody; Special AT rich sequence binding protein 2 antibody; Special AT-rich sequence-binding protein 2 antibody; anti-SATB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-SATB2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC/IF: 1:50-1:100
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC/IF: 1:50-1:100
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with SATB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with SATB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry)
(ICC staining SATB2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SATB2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SATB2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SATB2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SATB2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SATB2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of SATB2 on THP-1 cell using anti-SATB2 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of SATB2 on THP-1 cell using anti-SATB2 antibody at 1/500 dilution.)
Related Product Information for anti-SATB2 antibody
SATB2 (Special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.
Product Categories/Family for anti-SATB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
69,137 Da
NCBI Official Full Name
DNA-binding protein SATB2
NCBI Official Synonym Full Names
SATB homeobox 2
NCBI Official Symbol
SATB2
NCBI Official Synonym Symbols
GLSS
NCBI Protein Information
DNA-binding protein SATB2; SATB family member 2; special AT-rich sequence-binding protein 2
UniProt Protein Name
DNA-binding protein SATB2
UniProt Gene Name
SATB2
UniProt Synonym Gene Names
KIAA1034
UniProt Entry Name
SATB2_HUMAN
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Product Notes
The SATB2 satb2 (Catalog #AAA30388) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SATB2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SATB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC/IF: 1:50-1:100 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the SATB2 satb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SATB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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