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FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with SAMHD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Rabbit anti-Human SAMHD1 Monoclonal Antibody | anti-SAMHD1 antibody
SAMHD1 Antibody
Gene Names
SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
SAMHD1; Monoclonal Antibody; SAMHD1 Antibody; CHBL2 antibody; DCIP antibody; Dendritic cell derived IFNG induced protein antibody; Dendritic cell-derived IFNG-induced protein antibody; Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody; dNTPase antibody; HD domain containing 1 antibody; HDDC1 antibody; Mg11 antibody; Monocyte protein 5 antibody; MOP 5 antibody; MOP-5 antibody; MOP5 antibody; OTTHUMP00000030889 antibody; SAM domain and HD domain 1 antibody; SAM domain and HD domain containing protein 1 antibody; SAM domain and HD domain-containing protein 1 antibody; SAMH1_HUMAN antibody; Samhd1 antibody; SBBI88 antibody; anti-SAMHD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JU56-04
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-SAMHD1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with SAMHD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with SAMHD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry)
(ICC staining SAMHD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SAMHD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SAMHD1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SAMHD1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SAMHD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining SAMHD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1, 000 dilution.)
Related Product Information for anti-SAMHD1 antibody
SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.
Product Categories/Family for anti-SAMHD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 65; 69 kDa
Observed: 62 kDa
Observed: 62 kDa
NCBI Official Full Name
deoxynucleoside triphosphate triphosphohydrolase SAMHD1
NCBI Official Synonym Full Names
SAM domain and HD domain 1
NCBI Official Symbol
SAMHD1
NCBI Official Synonym Symbols
DCIP; CHBL2; HDDC1; MOP-5; SBBI88
NCBI Protein Information
deoxynucleoside triphosphate triphosphohydrolase SAMHD1; dNTPase; monocyte protein 5; dendritic cell-derived IFNG-induced protein; SAM domain and HD domain-containing protein 1
UniProt Protein Name
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
UniProt Gene Name
SAMHD1
UniProt Synonym Gene Names
MOP5; dNTPase; DCIP; MOP-5
UniProt Entry Name
SAMH1_HUMAN
Similar Products
Product Notes
The SAMHD1 samhd1 (Catalog #AAA30402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAMHD1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAMHD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the SAMHD1 samhd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAMHD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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