Rabbit RbAP48 Monoclonal Antibody | anti-RbAP48 antibody

RbAP48 Antibody

Cat. Num. AAA30415

• Gene Names: RBBP4; NURF55; RBAP48; lin-53
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: RbAP48, Antibody; RbAP48 Antibody; CAF I p48 antibody; CAF-1 subunit C antibody; CAF-I 48 kDa subunit antibody; CAF-I p48 antibody; Chromatin assembly factor 1 subunit C antibody; Chromatin assembly factor I p48 subunit antibody; Chromatin assembly factor/CAF 1 p48 subunit antibody; Histone-binding protein RBBP4 antibody; MSI1 protein homolog antibody; Nucleosome-remodeling factor subunit RBAP48 antibody; NURF55 antibody; RB binding protein 4 chromatin remodeling factor antibody; RbAp 48 antibody; RBAP48 antibody; RBBP-4 antibody; RBBP4 antibody; RBBP4_HUMAN antibody; Retinoblastoma binding protein 4 antibody; Retinoblastoma binding protein p48 antibody; Retinoblastoma-binding protein 4 antibody; Retinoblastoma-binding protein p48 antibody; anti-RbAP48 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: JU37-47
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-RbAP48 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC: 1:50-1:200; IP:1:10-1:50; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution. Positive control: Lane 1: NIH-3T3 Lane 2: Hela Lane 3: Rat brain Lane 4: Mouse testis)

Related Product Information for anti-RbAP48 antibody

In the intact cell, DNA is closely associated with histones and other nuclear proteins to form chromatin.The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation, and an increased accessiblity of DNA to transcription factors. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The retinoblastoma binding proteins RbAp46 and RbAp48 have been identified as histone binding proteins, and they are components of the histone deacetylase complex.

Product Categories/Family for anti-RbAP48 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 1172846
• NCBI GeneID: 5928
• UniProt Accession #: Q09028; P31149; Q53H02; Q96BV9; B2R6G9; B4DRH0; D3DPQ3
• Molecular Weight: 425
• NCBI Official Full Name: Histone-binding protein RBBP4
• NCBI Official Synonym Full Names: retinoblastoma binding protein 4
• NCBI Official Symbol: RBBP4
• NCBI Official Synonym Symbols: NURF55; RBAP48; lin-53
• NCBI Protein Information: histone-binding protein RBBP4; RBBP-4; CAF-I p48; CAF-1 subunit C; CAF-I 48 kDa subunit; MSI1 protein homolog; retinoblastoma-binding protein 4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 sub
• UniProt Protein Name: Histone-binding protein RBBP4
• UniProt Gene Name: RBBP4
• UniProt Synonym Gene Names: RBAP48; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; RBBP-4
• UniProt Entry Name: RBBP4_HUMAN