Human MYT1 ELISA Kit | MYT1 elisa kit

MYT1 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA30732

• Gene Names: PKMYT1; MYT1; PPP1R126
• Reactivity: Human
• Synonyms: MYT1; MYT1 Colorimetric Cell-Based ELISA Kit; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1 elisa kit

• Reactivity: Human
• Specificity: The Anti-MYT1 Antibody is a rabbit polyclonal antibody. It was tested on Western Blots for specificity The data in Figure 2 shows that a single protein band was detected. This protein band can be blocked by the synthesized immunogen peptide.
• Form/Format: One 96-Well Plate
• Sequence Length: 430
• Samples: Researchers plate their cell line of choice
• Assay Type: Non-Phospho Cell-Based ELISA
• Sensitivity: >=5000 cells/well

• Detection Method: Colorimetric 450 nm
• Preparation and Storage: Store at 4 degree C.

Related Product Information for MYT1 elisa kit

Background: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet wholecell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the nonphosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target

Principle of the Assay: The MYT1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MYT1 protein expression profile in cells. The kit can be used for measuring the relative amounts of MYT1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MYT1.

Product Categories/Family for MYT1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 386642875
• NCBI GeneID: 9088
• NCBI Accession #: NP_001245379.1
• NCBI GenBank Nucleotide #: NM_001258450.1
• UniProt Accession #: Q99640; O14731; Q7LE24; Q8TCM9; B3KUN8; B4DXD4; D3DUA4; F8W164; I3L1V2
• Molecular Weight: 54521
• NCBI Official Full Name: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 3
• NCBI Official Synonym Full Names: protein kinase, membrane associated tyrosine/threonine 1
• NCBI Official Symbol: PKMYT1
• NCBI Official Synonym Symbols: MYT1; PPP1R126
• NCBI Protein Information: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Gene Name: PKMYT1
• UniProt Synonym Gene Names: MYT1