Human, Mouse RIPK2 ELISA Kit | RIPK2 elisa kit
Phospho-RIPK2 (Ser176) Colorimetric Cell-Based ELISA Kit
Gene Names
RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
Reactivity
Human, Mouse
Synonyms
RIPK2; Phospho-RIPK2 (Ser176) Colorimetric Cell-Based ELISA Kit; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARDIAK; kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP-2; RIP-like interacting CLARP kinase; RIP2; RIPK2 elisa kit
Reactivity
Human, Mouse
Specificity
The Anti-RIPK2 (Phospho-Ser176) Antibody is a rabbit polyclonal antibody. It was tested on Western Blots for specificity. The data in Figure 2 shows that a single protein band was detected. This protein band can be blocked by the synthesized immunogen peptide.
Form/Format
Two 96-Well Plates
Sequence Length
540
Preparation and Storage
Store at 4 degree C.
Related Product Information for RIPK2 elisa kit
Background: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non- phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Principle of the Assay: The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can monitor RIPK2 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated RIPK2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 phosphorylation.
Principle of the Assay: The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can monitor RIPK2 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated RIPK2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 phosphorylation.
Product Categories/Family for RIPK2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61195
NCBI Official Full Name
receptor-interacting serine/threonine-protein kinase 2
NCBI Official Synonym Full Names
receptor interacting serine/threonine kinase 2
NCBI Official Symbol
RIPK2
NCBI Official Synonym Symbols
CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
NCBI Protein Information
receptor-interacting serine/threonine-protein kinase 2
UniProt Protein Name
Receptor-interacting serine/threonine-protein kinase 2
UniProt Gene Name
RIPK2
UniProt Synonym Gene Names
CARDIAK; RICK; RIP2; CARD-containing IL-1 beta ICE-kinase; RIP-2
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Product Notes
The Human, Mouse RIPK2 ripk2 (Catalog #AAA30733) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA30733 ELISA Kit recognizes Human, Mouse RIPK2. It is sometimes possible for the material contained within the vial of "RIPK2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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