Human C type lectin domain family 4 member D (CLEC4D) ELISA Kit | CLEC4D elisa kit

Human C type lectin domain family 4 member D (CLEC4D) ELISA Kit

Cat. Num. AAA27166

• Gene Names: CLEC4D; MCL; MPCL; CLEC6; CLEC-6; CLECSF8
• Reactivity: Human
• Synonyms: C type lectin domain family 4 member D (CLEC4D); N/A; Human C type lectin domain family 4 member D (CLEC4D) ELISA Kit; CLEC4D elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CLEC4D. No significant cross-reactivity or interference between CLEC4D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC4D and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CLEC4D elisa kit

Principle of the Assay: CLEC4D ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC4D antibody and an CLEC4D-HRP conjugate. The assay sample and buffer are incubated together with CLEC4D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC4D concentration since CLEC4D from samples and CLEC4D-HRP conjugate compete for the anti-CLEC4D antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC4D from the sample, fewer sites are left to bind CLEC4D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC4D concentration in each sample is interpolated from this standard curve.

Intended Uses: This CLEC4D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC4D. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for CLEC4D elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 31542313
• NCBI GeneID: 338339
• NCBI Accession #: NP_525126.2
• NCBI GenBank Nucleotide #: NM_080387.4
• Molecular Weight: 24,704 Da
• NCBI Official Full Name: C-type lectin domain family 4 member D
• NCBI Official Synonym Full Names: C-type lectin domain family 4, member D
• NCBI Official Symbol: CLEC4D
• NCBI Official Synonym Symbols: MCL; MPCL; CLEC6; CLEC-6; CLECSF8
• NCBI Protein Information: C-type lectin domain family 4 member D; C-type lectin receptor; macrophage C-type lectin; C-type lectin-like receptor 6; C-type lectin superfamily member 8; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 8
• UniProt Protein Name: C-type lectin domain family 4 member D
• UniProt Gene Name: CLEC4D
• UniProt Synonym Gene Names: CLECSF8; MCL; CLEC-6
• UniProt Entry Name: CLC4D_HUMAN