IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
Rabbit Akt Polyclonal Antibody | anti-Akt antibody
Akt (Phospho-Thr308) Rabbit Polyclonal Antibody
WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/20000
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,Akt (phospho Thr308) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Akt (T308) Polyclonal Antibody diluted at 1:500)
WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Akt (T308) Polyclonal Antibody diluted at 1:500)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
ELISA
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt (Phospho-Thr308) Antibody)
ELISA
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt (Phospho-Thr308) Antibody)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer, using Akt (Phospho-Thr308) Antibody. The picture on the right is blocked with the Akt (Phospho-Thr308) peptide.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer, using Akt (Phospho-Thr308) Antibody. The picture on the right is blocked with the Akt (Phospho-Thr308) peptide.)
WB (Western Blot)
(Western blot analysis of Akt (Phospho-Thr308) Antibody. The lane on the right is blocked with the Akt (Phospho-Thr308) peptide.)
WB (Western Blot)
(Western blot analysis of Akt (Phospho-Thr308) Antibody. The lane on the right is blocked with the Akt (Phospho-Thr308) peptide.)
NCBI and Uniprot Product Information
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Product Notes
The Akt akt1 (Catalog #AAA30742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Akt (Phospho-Thr308) Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Akt can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA). IF: 1:50-200 WB: 1/500-1/2000 IHC: 1/100-1/300 ELISA: 1/20000. Researchers should empirically determine the suitability of the Akt akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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