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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

Rabbit c-Src Polyclonal Antibody | anti-c-Src antibody

c-Src Rabbit Polyclonal Antibody

Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
c-Src; Polyclonal Antibody; c-Src Rabbit Polyclonal Antibody; SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; BH6842; anti-c-Src antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
c-Src Polyclonal Antibody detects endogenous levels of c-Src protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-c-Src antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC: 1/100-1/300
IF: 1/200-1/1000
ELISA: 1/40000
Immunogen
Synthesized peptide derived from c-Src. at AA range: 360-440
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

WB (Western Blot)

(Western Blot analysis of various cells using c-Src Polyclonal Antibody diluted at 1:2000)

WB (Western Blot) (Western Blot analysis of various cells using c-Src Polyclonal Antibody diluted at 1:2000)

WB (Western Blot)

(Western Blot analysis of 293 cells using c-Src Polyclonal Antibody diluted at 1:2000)

WB (Western Blot) (Western Blot analysis of 293 cells using c-Src Polyclonal Antibody diluted at 1:2000)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)
Related Product Information for anti-c-Src antibody
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,835 Da
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase Src
NCBI Official Synonym Full Names
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
NCBI Official Symbol
SRC
NCBI Official Synonym Symbols
ASV; SRC1; c-SRC; p60-Src
NCBI Protein Information
proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase Src
UniProt Gene Name
SRC
UniProt Synonym Gene Names
SRC1; p60-Src
UniProt Entry Name
SRC_HUMAN

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Product Notes

The c-Src src (Catalog #AAA30749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-Src Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-Src can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1/500-1/2000 IHC: 1/100-1/300 IF: 1/200-1/1000 ELISA: 1/40000. Researchers should empirically determine the suitability of the c-Src src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Src, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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