Mouse anti-Mouse, Rat HAO1 Monoclonal Antibody | anti-HAO1 antibody

HAO1 Monoclonal Antibody (Mix)

Cat. Num. AAA30820

• Gene Names: HAO1; GOX; GOX1; HAOX1
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Synonyms: HAO1; Monoclonal Antibody; HAO1 Monoclonal Antibody (Mix); Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX; anti-HAO1 antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Clone Number: Mix
• Specificity: The antibody detects endogenous HAO1 protein.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-HAO1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000-2000; IF: 1:200; IHC: 1:50-300
• Immunogen: Recombinant Protein of HAO1
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Human-appendix tissue. 1.HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1.Chk2 (phospho Thr68) Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). HAO1 Monoclonal Antibody(Mix)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1.HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

WB (Western Blot)

(Western blot analysis of 1) Mouse Liver Tissue. 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.)

Related Product Information for anti-HAO1 antibody

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]

Product Categories/Family for anti-HAO1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 11068137
• NCBI GeneID: 54363
• NCBI Accession #: NP_060015.1
• NCBI GenBank Nucleotide #: NM_017545.3
• UniProt Accession #: Q9UJM8
• NCBI Official Full Name: hydroxyacid oxidase 1
• NCBI Official Synonym Full Names: hydroxyacid oxidase 1
• NCBI Official Symbol: HAO1
• NCBI Official Synonym Symbols: GOX; GOX1; HAOX1
• NCBI Protein Information: hydroxyacid oxidase 1
• UniProt Protein Name: Hydroxyacid oxidase 1
• UniProt Gene Name: HAO1
• UniProt Synonym Gene Names: GOX1; HAOX1; HAOX1; GOX
• UniProt Entry Name: HAOX1_HUMAN