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IHC (Immunohistchemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Rabbit BCLAF1 Polyclonal Antibody | anti-BCLAF1 antibody
Phospho-BCLAF1 (Ser531) Antibody
Gene Names
BCLAF1; BTF; bK211L9.1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
BCLAF1; Polyclonal Antibody; Phospho-BCLAF1 (Ser531) Antibody; Bcl 2 associated transcription factor; Bcl-2-associated transcription factor 1; BCL2 associated transcription factor 1; BCLF1_HUMAN; bK211L9.1; Btf; KIAA0164; anti-BCLAF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-BCLAF1 (Ser531) Antibody detects endogenous levels of BCLAF1 only when phosphorylated at Ser531.
Tissue Specificity: Ubiquitous.
Tissue Specificity: Ubiquitous.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BCLAF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human BCLAF1 around the phosphorylation site of Ser531.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Citrullinated by PADI4.
Subunit Structure
Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19kDa protein and with DNA. Component of the SNARP complex which consists at least of SNIP1, SNW1, THRAP3, BCLAF1 and PNN. Component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, KIAA1429, RBM15, BCLAF1 and THRAP3.
Similarity
Belongs to the BCLAF1/THRAP3 family.
Subcellular Location
Cytoplasm. Nucleus. Nucleus speckle. Nucleus>Nucleoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistchemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistchemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of BCLAF1 (Phospho-Ser531) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of BCLAF1 (Phospho-Ser531) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of extracts from 293, using BCLAF1 (Phospho-Ser531) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using BCLAF1 (Phospho-Ser531) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse kidney, using Phospho-BCLAF1 (Ser531) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse kidney, using Phospho-BCLAF1 (Ser531) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-BCLAF1 antibody
Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106,122 Da
NCBI Official Full Name
bcl-2-associated transcription factor 1 isoform 2
NCBI Official Synonym Full Names
BCL2-associated transcription factor 1
NCBI Official Symbol
BCLAF1
NCBI Official Synonym Symbols
BTF; bK211L9.1
NCBI Protein Information
bcl-2-associated transcription factor 1
UniProt Protein Name
Bcl-2-associated transcription factor 1
UniProt Gene Name
BCLAF1
UniProt Synonym Gene Names
BTF; KIAA0164; Btf
UniProt Entry Name
BCLF1_HUMAN
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Product Notes
The BCLAF1 bclaf1 (Catalog #AAA31422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-BCLAF1 (Ser531) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's BCLAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the BCLAF1 bclaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCLAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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